Biotinylated Cynomolgus Alkaline Phosphatase (Placental type) /ALPP Protein (Primary Amine Labeling) (active dimer)

Name: Biotinylated Cynomolgus Alkaline Phosphatase (Placental type) /ALPP Protein (Primary Amine Labeling) (active dimer)
Brand: 普维拓生物
SKU: ALPP-CB001

货号ALPP-CB001

HEK293分子名 · Alkaline phosphatase理论分子量 · 53.69 kDa

产品简介

Recombinant Biotinylated Cynomolgus Alkaline Phosphatase (Placental type) /ALPP Protein (Primary Amine Labeling) is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Ile21-Thr503 (accession: XP_045223825.1).

产品详情

分子别名: Alkaline phosphatase; PLAP-1; ALPP; PLAP
蛋白长度: Ile21-Thr503
表达系统: HEK293
理论分子量: The protein has a predicted MW of 53.69 kDa. Due to glycosylation, the protein migrates to 57-70 kDa based on Bis-Tris PAGE result.
纯度: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
内毒素: Less than 1 EU per μg by the LAL method.
缓冲液 / 制剂: Lyophilized from 0.22 μm filtered solution in 25mM Tris, 100mM NaCl, 1mM MgCl 2 (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
状态: 冻干粉
储存条件: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
复溶建议: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

数据展示

Bis-Tris PAGE Data — Biotinylated Cynomolgus ALPP on Bis-Tris PAGE under reduced (R) condition and Non-reduced (N) condition. The purity is greater than 95%..

SEC-HPLC Data — The purity of Biotinylated Cynomolgus ALPP is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Biotinylated Cynomolgus ALPP, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-ALPP Antibody, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA. (QC Test)

SPR Data — Anti-Alkaline Phosphatase Antibody captured on CM5 Chip via Protein A can bind Biotinylated Cynomolgus ALPP, His Tag with an affinity constant of 82.54 nM as determined in SPR assay (Biacore T200).

BLI Data — Loaded Anti-Alkaline Phosphatase Antibody, hFc Tag on ProA-Biosensor can bind Biotinylated Cynomolgus ALPP, His Tag with an affinity constant of 0.13 µM as determined in BLI assay.

背景介绍

Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease.

文献引用

1.Siller AF, Whyte MP. Alkaline Phosphatase: Discovery and Naming of Our Favorite Enzyme. J Bone Miner Res. 2018 Feb;33(2):362-364. doi: 10.1002/jbmr.3225. Epub 2017 Aug
2.PMID:
3.Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.

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Biotinylated Cynomolgus Alkaline Phosphatase (Placental type) /ALPP Protein (Primary Amine Labeling) (active dimer)

货号ALPP-CB001

HEK293分子名 · Alkaline phosphatase理论分子量 · 53.69 kDa

Biotinylated Cynomolgus Alkaline Phosphatase (Placental type) /ALPP Protein (Primary Amine Labeling) (active dimer)

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

SPR

SPR Data

BLI

BLI Data

背景介绍

文献引用

Biotinylated Cynomolgus Alkaline Phosphatase (Placental type) /ALPP Protein (Primary Amine Labeling) (active dimer)

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

SPR

SPR Data

BLI

BLI Data

背景介绍

文献引用