Biotinylated Cynomolgus Alkaline Phosphatase (Placental type) /ALPP Protein (Primary Amine Labeling) (active dimer)

Name: Biotinylated Cynomolgus Alkaline Phosphatase (Placental type) /ALPP Protein (Primary Amine Labeling) (active dimer)
Brand: Puweituo Bio
SKU: ALPP-CB001

Catalog numberALPP-CB001

HEK293Molecule · Alkaline phosphataseMolecular weight · 53.69 kDa

Product introduction

Recombinant Biotinylated Cynomolgus Alkaline Phosphatase (Placental type) /ALPP Protein (Primary Amine Labeling) is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Ile21-Thr503 (accession: XP_045223825.1).

Product details

Molecular aliases: Alkaline phosphatase; PLAP-1; ALPP; PLAP
Protein length: Ile21-Thr503
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 53.69 kDa. Due to glycosylation, the protein migrates to 57-70 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22 μm filtered solution in 25mM Tris, 100mM NaCl, 1mM MgCl 2 (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Biotinylated Cynomolgus ALPP on Bis-Tris PAGE under reduced (R) condition and Non-reduced (N) condition. The purity is greater than 95%..

SEC-HPLC Data — The purity of Biotinylated Cynomolgus ALPP is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Biotinylated Cynomolgus ALPP, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-ALPP Antibody, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA. (QC Test)

SPR Data — Anti-Alkaline Phosphatase Antibody captured on CM5 Chip via Protein A can bind Biotinylated Cynomolgus ALPP, His Tag with an affinity constant of 82.54 nM as determined in SPR assay (Biacore T200).

BLI Data — Loaded Anti-Alkaline Phosphatase Antibody, hFc Tag on ProA-Biosensor can bind Biotinylated Cynomolgus ALPP, His Tag with an affinity constant of 0.13 µM as determined in BLI assay.

Background

Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease.

References

1.Siller AF, Whyte MP. Alkaline Phosphatase: Discovery and Naming of Our Favorite Enzyme. J Bone Miner Res. 2018 Feb;33(2):362-364. doi: 10.1002/jbmr.3225. Epub 2017 Aug
2.PMID:
3.Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.

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Biotinylated Cynomolgus Alkaline Phosphatase (Placental type) /ALPP Protein (Primary Amine Labeling) (active dimer)

Catalog numberALPP-CB001

HEK293Molecule · Alkaline phosphataseMolecular weight · 53.69 kDa

Biotinylated Cynomolgus Alkaline Phosphatase (Placental type) /ALPP Protein (Primary Amine Labeling) (active dimer)

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

SPR

SPR Data

BLI

BLI Data

Background

References

Biotinylated Cynomolgus Alkaline Phosphatase (Placental type) /ALPP Protein (Primary Amine Labeling) (active dimer)

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

SPR

SPR Data

BLI

BLI Data

Background

References