PHASE 01
Assessment
- 01
Sequence analysis and project design
Review domains, signal peptides, transmembrane regions, modifications and stability to define construct, tag and purification strategy.
DeliverableProject and construct plan
RECOMBINANT PROTEIN PRODUCTION
Protein expression and production
PROVETOP provides protein expression and production services for universities, research institutes, biopharma companies and diagnostic developers.
We provide an integrated route from sequence analysis and system selection through vector construction, pilot expression, purification optimization and quality testing.
Service advantages
Samples designed for final use
Sample usability
Multiple expression platforms
Bacterial, mammalian and insect-cell platforms are matched to structure, modification, yield and intended use.
Designed around downstream use
Tag position, construct, buffer and quality criteria are designed around research, antibody, screening or diagnostic use.
Experience with difficult proteins
Multi-route strategies address low expression, degradation, aggregation, disulfide bonds, glycosylation and complex domains.
Integrated expression, purification and testing
One technical chain connects gene to sample with clear stage feedback and fewer handoff risks.
Fit-for-purpose quality criteria
Purity, endotoxin, aggregation, activity and other tests are configured around intended use.
From pilot to scale-up
Microgram- and milligram-scale research production can progress into process optimization and scale-up.
Service process
The workflow and milestones depend on the expression system, construct complexity, purification challenge and analytical requirements, and are documented after technical review.
PHASE 02
Design & expression
- 02
Gene synthesis and vector construction
Complete codon optimization, gene synthesis, expression-vector construction and sequence confirmation.
DeliverableConstruct and sequence confirmation - 03
Pilot expression and condition screening
Assess expression, solubility, secretion and stability; optimize host, tag and culture conditions for difficult targets.
DeliverablePilot results and condition recommendations
PHASE 03
Purification
- 04
Scale-up expression and purification
Scale culture, harvest and purify through multiple steps, with buffer and storage optimization as needed.
DeliverablePurified sample and process records - 05
Quality testing and sample delivery
Complete agreed purity, concentration, molecular-weight, endotoxin, aggregation or activity tests and deliver sample and report.
DeliverableTest results and sample delivery
PHASE 04
Evidence & delivery
- 06
Project records and handling guidance
Deliver the project report, agreed test results, and guidance for storage, aliquoting and use.
DeliverableDelivered with the sample
Service scope & deliverables
Scope and delivery
Expression platform, target type, quality testing and deliverables are configured around protein properties and intended use.
Service moduleTechnical scopeExecution focusFinal deliverable
Multi-platform protein expression
Technical scope
Bacterial, mammalian, insect-cell and other custom expression routes.
Execution focus
Match platform to molecular size, domains, transmembrane regions, signal peptides, disulfides and modification needs.
Final deliverable
Expression plan and purified sample
Diverse and difficult protein targets
Technical scope
Cytokines, receptor domains, antigens, enzymes, fusion and multidomain proteins, glycoproteins and membrane targets.
Execution focus
Use multi-construct and multi-system strategies for low expression, degradation, aggregation and complex folding.
Final deliverable
Target protein fit for agreed use
Quality testing and project delivery
Technical scope
Configurable SDS-PAGE, concentration, molecular weight, Western blot, SEC, endotoxin, microbial and activity testing.
Execution focus
Testing is selected by protein properties and intended use, with storage, aliquoting and handling considered.
Final deliverable
Protein sample, test results and project report
Case studies
Typical project examples show how expression systems are selected, technical challenges are addressed and deliverables are formed.
Complex glycoprotein in mammalian cells
PROJECT NEED
Produce a human receptor extracellular domain with glycosylation sites and disulfide bonds for antibody screening and interaction studies.
TECHNICAL ROUTE
Define domain boundaries, design a secreted construct, use transient mammalian expression, and remove aggregates through affinity and size-exclusion purification.
PROJECT RESULT
A high-purity, homogeneous target protein suitable for downstream antibody binding and molecular-interaction studies.
Low-solubility antigen in bacterial expression
PROJECT NEED
The original route produced low expression and inclusion bodies; an antigen suitable for immunization was required.
TECHNICAL ROUTE
Adjust construct boundaries and tag position, screen strain and induction conditions, and compare soluble expression with inclusion-body refolding.
PROJECT RESULT
Soluble expression improved and the purified material met downstream immunization needs.
Cytokine production and activity confirmation
PROJECT NEED
Produce an active recombinant cytokine for cell culture and in-vitro functional studies with endotoxin control.
TECHNICAL ROUTE
Use a eukaryotic secretion system, multi-step purification, endotoxin testing and cell-based activity confirmation.
PROJECT RESULT
The delivered sample met agreed purity, endotoxin and biological-activity requirements for downstream cell studies.
Frequently asked questions
Clarify these questions before assessment and project execution.
Can we start with only a protein name and no final construct?
Yes. Provide the species, intended use and known structural information so construct boundaries can be discussed during feasibility review.
How is the expression system selected?
Selection considers size, modification needs, structural complexity, intended use and budget, with alternatives retained for higher-risk targets.
Can you help optimize the sequence or tag design?
We can discuss domain boundaries, signal peptides, tag placement and purification needs. Designs involving functional sites or intellectual property require joint confirmation.
What information is needed before project start?
Typically we need the target sequence or accession, species, desired format, downstream use, expected sample form and known risk information.
Is the quality-test package fixed?
No. Baseline tests and additional analyses are agreed at project start according to protein properties, sample amount and intended use.
Project consultation
Start your protein expression project
Send the target sequence, intended use, purity, quantity and testing requirements for an initial assessment.