Human TNFSF15 (R32A, R85A) Trimer Protein

Name: Human TNFSF15 (R32A, R85A) Trimer Protein
Brand: 普维拓生物
SKU: TNFSF15-HP004

货号TNFSF15-HP004

HumanHEK293分子名 · TL1A理论分子量 · 57.64 kDa

产品简介

Recombinant Human TNFSF15 (R32A, R85A) Trimer Protein is expressed in HEK293 cells with a His tag and Flag tag at the N-terminus. It contains amino acid residues Asp91-Leu251 trimer design, the R32A and R85A mutations correspond to positions 103 and 156 in the UniProt sequence (UniProt accession: O95150).

产品详情

分子别名: TL1A; VEGI-251; TNFSF15; TL1; VEGI; VEGI192A; VEGI251
蛋白长度: Asp91-Leu251
表达系统: HEK293
理论分子量: The protein has a predicted MW of 57.64 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Bis-Tris PAGE result.
纯度: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
内毒素: Less than 0.1 EU per μg by the LAL method.
缓冲液 / 制剂: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
状态: 冻干粉
储存条件: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
复溶建议: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

数据展示

Bis-Tris PAGE Data — Human TNFSF15 (R32A, R85A) Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Human TNFSF15 (R32A, R85A) Trimer is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Human TNFSF15 (R32A, R85A) Trimer, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA. (QC Test)

背景介绍

TNF superfamily member 15 (TNFSF15), a cytokine largely produced by vascular endothelial cells and a specific inhibitor of the proliferation of these same cells, can inhibit VEGF-induced vascular permeability in vitro and in vivo, and that death receptor 3 (DR3), a cell surface receptor of TNFSF15, mediates TNFSF15-induced dephosphorylation of VEGFR2.

文献引用

1.Valatas V, Kolios G, Bamias G. TL1A (TNFSF15) and DR3 (TNFRSF25): A Co-stimulatory System of Cytokines With Diverse Functions in Gut Mucosal Immunity. Front Immunol. 2019;10:583. Published 2019 Mar 27. doi:10.3389/fimmu.2019.00583

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Human TNFSF15 (R32A, R85A) Trimer Protein

货号TNFSF15-HP004

HumanHEK293分子名 · TL1A理论分子量 · 57.64 kDa

Human TNFSF15 (R32A, R85A) Trimer Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

背景介绍

文献引用

Human TNFSF15 (R32A, R85A) Trimer Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

背景介绍

文献引用