Cynomolgus/Rhesus macaque TNFSF15 Protein

Name: Cynomolgus/Rhesus macaque TNFSF15 Protein
Brand: 普维拓生物
SKU: TNFSF15-CP002

货号TNFSF15-CP002

HEK293分子名 · TL1A理论分子量 · 44.82 kDa

产品简介

Recombinant Cynomolgus/Rhesus macaque TNFSF15 Protein is expressed in HEK293 cells with monomeric Fc tag at the N-terminus. It contains amino acid residues Leu72-Leu251 (accession: A0A2K5UA22(Cynomolgus) / F6S8F9(Rhesus macaque)).

产品详情

分子别名: TL1A; VEGI-251; TNFSF15; TL1; VEGI; VEGI192A; TNF superfamily member 15; VEGI251
蛋白长度: Leu72-Leu251
表达系统: HEK293
理论分子量: The protein has a predicted MW of 44.82 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.
纯度: > 95% as determined by Bis-Tris PAGE > 85% as determined by HPLC
内毒素: Less than 1 EU per μg by the LAL method.
缓冲液 / 制剂: Lyophilized from 0.22 μm filtered solution in PBS, 8% trehalose (pH 7.4).
状态: 冻干粉
储存条件: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
复溶建议: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

数据展示

Bis-Tris PAGE Data — Cynomolgus/Rhesus macaque TNFSF15 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Cynomolgus/Rhesus macaque TNFSF15 is greater than 85% as determined by SEC-HPLC.

SEC-MALS Data — The purity of Cynomolgus/Rhesus macaque TNFSF15 is greater than 85% and the molecular weight of this protein is around 140-180 kDa as determined by SEC-MALS.

ELISA Data — Immobilized Mouse DR3, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Cynomolgus/Rhesus macaque TNFSF15, hFc Tag with the EC50 of 27.1ng/ml determined by ELISA (QC Test).

背景介绍

TNF superfamily member 15 (TNFSF15), a cytokine largely produced by vascular endothelial cells and a specific inhibitor of the proliferation of these same cells, can inhibit VEGF-induced vascular permeability in vitro and in vivo, and that death receptor 3 (DR3), a cell surface receptor of TNFSF15, mediates TNFSF15-induced dephosphorylation of VEGFR2.

文献引用

1.Valatas V, Kolios G, Bamias G. TL1A (TNFSF15) and DR3 (TNFRSF25): A Co-stimulatory System of Cytokines With Diverse Functions in Gut Mucosal Immunity. Front Immunol. 2019;10:583. Published 2019 Mar 27. doi:10.3389/fimmu.2019.00583

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Cynomolgus/Rhesus macaque TNFSF15 Protein

货号TNFSF15-CP002

HEK293分子名 · TL1A理论分子量 · 44.82 kDa

Cynomolgus/Rhesus macaque TNFSF15 Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

SEC-MALS

SEC-MALS Data

ELISA

ELISA Data

ELISA

ELISA Data

背景介绍

文献引用

Cynomolgus/Rhesus macaque TNFSF15 Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

SEC-MALS

SEC-MALS Data

ELISA

ELISA Data

ELISA

ELISA Data

背景介绍

文献引用