Biotinylated Human HLA-A*02:01&B2M&TdT (ALYDKTKRIFL) Monomer Protein

Name: Biotinylated Human HLA-A*02:01&B2M&TdT (ALYDKTKRIFL) Monomer Protein
Brand: 普维拓生物
SKU: TDT-HB001

货号TDT-HB001

HumanHEK293分子名 · DNA nucleotidylexotransferase理论分子量 · 50.80 kDa

产品简介

Recombinant Biotinylated Human HLA-A*02:01&B2M&TdT (ALYDKTKRIFL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and ALYDKTKRIFL peptide (accession: A0A140T913(HLA-A*02:01) & P61769(B2M) &ALYDKTKRIFL).

产品详情

分子别名: DNA nucleotidylexotransferase; terminal deoxynucleotidyl transferase
蛋白长度: Gly25-Thr305
表达系统: HEK293
理论分子量: The protein has a predicted MW of 50.80 kDa. Due to glycosylation, the protein migrates to 52-65 kDa based on Bis-Tris PAGE result.
纯度: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
内毒素: Less than 1 EU per μg by the LAL method.
缓冲液 / 制剂: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
状态: 冻干粉
储存条件: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
复溶建议: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

数据展示

Bis-Tris PAGE Data — Biotinylated Human HLA-A*02:01&B2M&TdT (ALYDKTKRIFL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Human HLA-A*02:01&B2M&TdT (ALYDKTKRIFL) Monomer is greater than 95% as determined by SEC-HPLC.

背景介绍

The terminal deoxynucleotidyl transferase (TdT) belongs to the X family of DNA polymerases. This unusual polymerase catalyzes the template-independent addition of random nucleotides on 3'-overhangs during V(D)J recombination. The biological function and intrinsic biochemical properties of the TdT have spurred the development of numerous oligonucleotide-based tools and methods, especially if combined with modified nucleoside triphosphates.

文献引用

1.Sarac I, Hollenstein M. Terminal Deoxynucleotidyl Transferase in the Synthesis and Modification of Nucleic Acids. Chembiochem. 2019 Apr 1;20(7):860-871. doi: 10.1002/cbic.201800658. Epub 2019 Jan 25. PMID: 30451377.

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Biotinylated Human HLA-A*02:01&B2M&TdT (ALYDKTKRIFL) Monomer Protein

货号TDT-HB001

HumanHEK293分子名 · DNA nucleotidylexotransferase理论分子量 · 50.80 kDa

Biotinylated Human HLA-A*02:01&B2M&TdT (ALYDKTKRIFL) Monomer Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

背景介绍

文献引用

Biotinylated Human HLA-A*02:01&B2M&TdT (ALYDKTKRIFL) Monomer Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

背景介绍

文献引用