Human HLA-B*15:01&B2M&SARS-CoV-2 epitope (NQKLIANQF) Monomer Protein

Name: Human HLA-B*15:01&B2M&SARS-CoV-2 epitope (NQKLIANQF) Monomer Protein
Brand: 普维拓生物
SKU: SCOV2EPI-HP001

货号SCOV2EPI-HP001

HumanHEK293分子名 · MHC; SARS-CoV-2 epitope;理论分子量 · 50.50 kDa

产品简介

Recombinant Human HLA-B*15:01&B2M&SARS-CoV-2 epitope (NQKLIANQF) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains Gly21-Thr301(HLA-B*15:01), Ile21-Met119(B2M) and NQKLIANQF peptide (accession: AAA53258.1(HLA-B*15:01) & P61769(B2M) &NQKLIANQF).

产品详情

分子别名: MHC; SARS-CoV-2 epitope;
蛋白长度: Gly21-Thr301
表达系统: HEK293
理论分子量: The protein has a predicted MW of 50.50 kDa. Due to glycosylation, the protein migrates to 52-68 kDa based on Bis-Tris PAGE result.
纯度: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
内毒素: Less than 1 EU per μg by the LAL method.
缓冲液 / 制剂: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
状态: 冻干粉
储存条件: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
复溶建议: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

数据展示

Bis-Tris PAGE Data — Human HLA-B*15:01&B2M&SARS-CoV-2 epitope (NQKLIANQF) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Human HLA-B*15:01&B2M&SARS-CoV-2 epitope (NQKLIANQF) Monomer is greater than 95% as determined by SEC-HPLC.

背景介绍

HLA-B*15:01 is strongly associated with asymptomatic infection with SARS-CoV-2 and is likely to be involved in the mechanism underlying early viral clearance. T cells from pre-pandemic individuals carrying HLA-B*15:01 were reactive to the immunodominant SARS-CoV-2 S-derived peptide NQKLIANQF, and 100% of the reactive cells displayed memory phenotype.

文献引用

1.[1] Augusto DG, Yusufali T, Sabatino JJ Jr, Peyser ND, Murdolo LD, Butcher X, Murray V, Pae V, Sarvadhavabhatla S, Beltran F, Gill G, Lynch K, Yun C, Maguire C, Peluso MJ, Hoh R, Henrich TJ, Deeks SG, Davidson M, Lu S, Goldberg SA, Kelly JD, Martin JN, Viera-Green CA, Spellman SR, Langton DJ, Lee S, Marcus GM, Olgin JE, Pletcher MJ, Gras S, Maiers M, Hollenbach JA. A common allele of HLA mediates asymptomatic SARS-CoV-2 infection. medRxiv [Preprint]. 2022 Oct 12:2021.05.13.21257065.
2.[2] Augusto DG, Hollenbach JA. HLA variation and antigen presentation in COVID-19 and SARS-CoV-2 infection. Curr Opin Immunol. 2022 Jun;76:102178.

加载中…

Human HLA-B*15:01&B2M&SARS-CoV-2 epitope (NQKLIANQF) Monomer Protein

货号SCOV2EPI-HP001

HumanHEK293分子名 · MHC; SARS-CoV-2 epitope;理论分子量 · 50.50 kDa

Human HLA-B*15:01&B2M&SARS-CoV-2 epitope (NQKLIANQF) Monomer Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

背景介绍

文献引用

Human HLA-B*15:01&B2M&SARS-CoV-2 epitope (NQKLIANQF) Monomer Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

背景介绍

文献引用