Biotinylated SARS-COV-2 Spike S1 (Omicron B.1.1.529) Protein

Name: Biotinylated SARS-COV-2 Spike S1 (Omicron B.1.1.529) Protein
Brand: 普维拓生物
SKU: SARS-HB005

货号SARS-HB005

HEK293分子名 · S1 protein理论分子量 · 77.94 kDa

产品简介

Recombinant Biotinylated SARS-CoV-2 Spike S1 (Omicron B.1.1.529) Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Gln14-Arg683(A67V, HV69-70del, T95I, G142D, VYY143-145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H) (accession: QHO60594.1).

产品详情

分子别名: S1 protein; Spike protein S1; Spike,S1 protein; S glycoprotein Subunit1
蛋白长度: Gln14-Arg683
表达系统: HEK293
理论分子量: The protein has a predicted MW of 77.94 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Bis-Tris PAGE result.
纯度: > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
内毒素: Less than 1 EU per μg by the LAL method.
缓冲液 / 制剂: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% saccharose is added as protectant before lyophilization.
状态: 冻干粉
储存条件: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
复溶建议: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

数据展示

Bis-Tris PAGE Data — Biotinylated SARS-CoV-2 Spike S1 (Omicron B.1.1.529) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated SARS-CoV-2 Spike S1 (Omicron B.1.1.529) is greater than 90% as determined by SEC-HPLC.

ELISA Data — Immobilized Biotinylated Biotinylated SARS-CoV-2 Spike S1 (Omicron B.1.1.529), His Tag at 1μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 0.35μg/ml determined by ELISA.

背景介绍

The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

文献引用

1.Belouzard S, Chu V C, Whittaker G R. Activation Of The Sars Coronavirus Spike Protein Via Sequential Proteolytic Cleavage At Two Distinct Sites[J]. Proceedings of the National Academy of Sciences of the United States of America, 2009, 106(14):5871-5876.

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Biotinylated SARS-COV-2 Spike S1 (Omicron B.1.1.529) Protein

货号SARS-HB005

HEK293分子名 · S1 protein理论分子量 · 77.94 kDa

Biotinylated SARS-COV-2 Spike S1 (Omicron B.1.1.529) Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

背景介绍

文献引用

Biotinylated SARS-COV-2 Spike S1 (Omicron B.1.1.529) Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

背景介绍

文献引用