Biotinylated Human HLA-A*02:01&B2M&P53 WT (HMTEVVRRC) Monomer Protein

Name: Biotinylated Human HLA-A*02:01&B2M&P53 WT (HMTEVVRRC) Monomer Protein
Brand: 普维拓生物
SKU: P53WT-HB001

货号P53WT-HB001

HumanE. coli分子名 · MHC理论分子量 · 35.6 kDa

产品简介

Recombinant Biotinylated Human HLA-A*02:01&B2M&P53 WT (HMTEVVRRC) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and HMTEVVRRC peptide (accession: A0A140T913(HLA-A*02:01) & P61769(B2M) &HMTEVVRRC).

产品详情

分子别名: MHC; HLA-A; P53; TP53; Antigen NY-CO-13; BCC7; FLJ92943; LFS1; TRP53
蛋白长度: Gly25-Thr305
表达系统: E. coli
理论分子量: The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
纯度: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
内毒素: Less than 1 EU per μg by the LAL method.
缓冲液 / 制剂: Supplied as 0.22 μm filtered solution in 20mM Tris, 200mM NaCl (pH 8.0).
储存条件: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

数据展示

Bis-Tris PAGE Data — Biotinylated Human HLA-A*02:01&B2M&P53 WT (HMTEVVRRC) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Human HLA-A*02:01&B2M&P53 WT (HMTEVVRRC) Monomer is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Biotinylated Human HLA-A*02:01&B2M&P53 WT (HMTEVVRRC) Monomer, His-Avi tag at 1μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 14.8ng/ml determined by ELISA.

背景介绍

p53 is a tumor suppressor protein. Under stressful conditions, p53 tightly regulates cell growth by promoting apoptosis and DNA repair. When p53 becomes mutated, it loses its function, resulting in abnormal cell proliferation and tumor progression. Depending on the p53 mutation, it has been shown to form aggregates leading to negative gain of function of the protein. p53 mutant associated aggregation has been observed in several cancer tissues and has been shown to promote tumor growth.

文献引用

1.(1)Donnellan Z, Bossi G, Harper J, et al. ImmTACs: bi-specific TCR-anti-CD3 fusions for targeted tumour killing[J]. Journal for ImmunoTherapy of Cancer, 2015, 3(Suppl 2):P299.

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Biotinylated Human HLA-A*02:01&B2M&P53 WT (HMTEVVRRC) Monomer Protein

货号P53WT-HB001

HumanE. coli分子名 · MHC理论分子量 · 35.6 kDa

Biotinylated Human HLA-A*02:01&B2M&P53 WT (HMTEVVRRC) Monomer Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

背景介绍

文献引用

Biotinylated Human HLA-A*02:01&B2M&P53 WT (HMTEVVRRC) Monomer Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

背景介绍

文献引用