FITC-Labeled Human MSLN/Mesothelin Protein

Name: FITC-Labeled Human MSLN/Mesothelin Protein
Brand: 普维拓生物
SKU: MSLN-HP001

货号MSLN-HP001

HumanHEK293分子名 · MSLN理论分子量 · 35.2 kDa

产品简介

Recombinant FITC-Labeled Human MSLN/Mesothelin Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Glu296-Gly580 (UniProt accession: Q13421-2).

产品详情

分子别名: MSLN; CAK1; Mesothelin; MPF; MPFSMRP; SMR
蛋白长度: Glu296-Gly580
表达系统: HEK293
理论分子量: The protein has a predicted MW of 35.2 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
纯度: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
缓冲液 / 制剂: Supplied as 0.22μm filtered solution in PBS (pH 7.4).
储存条件: Valid for 6 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

数据展示

Bis-Tris PAGE Data — FITC-Labeled Human MSLN on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of FITC-Labeled Human MSLN is greater than 95% as determined by SEC-HPLC.

Flow Cytometry Data — FACS Analysis of Anti-MSLN CAR Expression. 293T cells were transfected with anti-MSLN-scFv and His tag. Cells were stained with 5μg/ml FITC-Labeled Human MSLN (296-580), His Tag and FITC-labeled protein control. Non-transfected 293T cells and FITC-labeled protein control were used as negative control.

背景介绍

Mesothelin, also known as MSLN, is a protein that in humans is encoded by the MSLN gene.Cloning studies showed that the mesothelin gene encodes a precursor protein that is processed to yield mesothelin which is attached to the cell membrane by a glycophosphatidylinositol linkage and a 31-kDa shed fragment named megakaryocyte-potentiating factor (MPF). Although it has been proposed that mesothelin may be involved in cell adhesion, its biological function is not known.A knockout mouse line that lacks mesothelin reproduces and develops normally.

文献引用

1.Adusumilli P S, Cherkassky L, Villena-Vargas J, et al. Regional delivery of mesothelin-targeted CAR T cell therapy generates potent and long-lasting CD4-dependent tumor immunity[J]. Science Translational Medicine, 2014, 6(261):261ra151-261ra151.
2.Morello A, Sadelain M, Adusumilli P S. Mesothelin-Targeted CARs: Driving T cells to Solid Tumors[J]. Cancer Discovery, 2015, 6(2).

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FITC-Labeled Human MSLN/Mesothelin Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

Flow Cytometry

Flow Cytometry Data

背景介绍

文献引用

FITC-Labeled Human MSLN/Mesothelin Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

Flow Cytometry

Flow Cytometry Data

背景介绍

文献引用