Human HLA-A*11:01&B2M&KRAS G12V (VVGAVGVGK) Monomer Protein

Name: Human HLA-A*11:01&B2M&KRAS G12V (VVGAVGVGK) Monomer Protein
Brand: 普维拓生物
SKU: KRASG12V-HP005

货号KRASG12V-HP005

HumanE. coli分子名 · MHC理论分子量 · 35.36 kDa

产品简介

Recombinant Human HLA-A*11:01&B2M&KRAS G12V (VVGAVGVGK) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305(HLA-A*11:01), Ile21-Met119(B2M) and VVGAVGVGK peptide (accession: AAV53343.1(HLA-A*11:01) & P61769(B2M) &VVGAVGVGK).

产品详情

分子别名: MHC; KRAS; K-Ras 2; KRAS2; C-K-RAS; CFC2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KRAS1; KRAS2; NS; NS3; RASK2; GTPase Kras; KI-RAS; RALD
蛋白长度: Gly25-Thr305
表达系统: E. coli
理论分子量: The protein has a predicted MW of 35.36 kDa (HLA-A*11:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
纯度: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
内毒素: Less than 1 EU per μg by the LAL method.
缓冲液 / 制剂: Supplied as 0.22 μm filtered solution in 20mM Tris, 200mM NaCl (pH 8.0).
储存条件: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

数据展示

Bis-Tris PAGE Data — Human HLA-A*11:01&B2M&KRAS G12V (VVGAVGVGK) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Human HLA-A*11:01&B2M&KRAS G12V (VVGAVGVGK) Monomer is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Human HLA-A*11:01&B2M&KRAS G12V (VVGAVGVGK) Monomer, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 6.9ng/ml determined by ELISA (QC Test).

背景介绍

Kirsten rat sarcoma 2 viral oncogene homolog (KRAS) is the most commonly mutated oncogene in human cancer. The developments of many cancers depend on sustained expression and signaling of KRAS, which makes KRAS a high-priority therapeutic target. The virtual screening approach to discover novel KRAS inhibitors and synthetic lethality interactors of KRAS are discussed in detail.

文献引用

1.(1) Wu HZ, Xiao JQ, Xiao SS, Cheng Y. KRAS: A Promising Therapeutic Target for Cancer Treatment. Curr Top Med Chem. 2019;19(23):2081-2097. doi: 10.2174/1568026619666190905164144. PMID: 31486755.

加载中…

Human HLA-A*11:01&B2M&KRAS G12V (VVGAVGVGK) Monomer Protein

货号KRASG12V-HP005

HumanE. coli分子名 · MHC理论分子量 · 35.36 kDa

Human HLA-A*11:01&B2M&KRAS G12V (VVGAVGVGK) Monomer Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

背景介绍

文献引用

Human HLA-A*11:01&B2M&KRAS G12V (VVGAVGVGK) Monomer Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

背景介绍

文献引用