Human IL-7 Protein

Name: Human IL-7 Protein
Brand: 普维拓生物
SKU: IL7-HP001

货号IL7-HP001

HumanE. coli分子名 · IL7理论分子量 · 17.5 kDa

产品简介

Recombinant Human IL-7 Protein is expressed in E. coli without tag. It contains amino acid residues Asp26-His177 (UniProt accession: P13232).

产品详情

分子别名: IL7; Interleukin-7; IL-7; Lymphopoietin-1; PBGF
蛋白长度: Asp26-His177
表达系统: E. coli
理论分子量: The protein has a predicted MW of 17.5 kDa same as Bis-Tris PAGE result.
纯度: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
内毒素: Less than 0.01 EU per μg by the LAL method.
缓冲液 / 制剂: Lyophilized from 0.22 μm filtered solution in PBS (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
状态: 冻干粉
储存条件: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
复溶建议: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

数据展示

Bis-Tris PAGE Data — Human IL-7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Human IL-7 is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Human IL-7 at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-7R alpha, hFc Tag with the EC50 of 42.1ng/ml determined by ELISA (QC tested).

SPR Data — Human IL-7R alpha, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-7, No Tag with an affinity constant of 35.38 nM as determined in SPR assay (Biacore S200).

Cell Proliferation Bioactivity — Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL). The ED50 for this effect is 0.100-1 ng/mL. (QC Test)

背景介绍

Interleukin-7 (IL7) plays a nonredundant role in T cell survival and homeostasis, which is illustrated in the severe T cell lymphopenia of IL7-deficient mice, or demonstrated in animals or humans that lack expression of either the IL7Rα or γ c chain, the two subunits that constitute the functional IL7 receptor.

文献引用

1.Park JH, Waickman AT, Reynolds J, Castro M, Molina-París C. IL7 receptor signaling in T cells: A mathematical modeling perspective. Wiley Interdiscip Rev Syst Biol Med. 2019;11(5):e1447. doi:10.1002/wsbm.1447

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Human IL-7 Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

SPR

SPR Data

Bioactivity

Cell Proliferation Bioactivity

背景介绍

文献引用

Human IL-7 Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

SPR

SPR Data

Bioactivity

Cell Proliferation Bioactivity

背景介绍

文献引用