Human IgG4 Fc Protein

Name: Human IgG4 Fc Protein
Brand: 普维拓生物
SKU: IGG4FC-HP001

货号IGG4FC-HP001

HumanHEK293分子名 · IGHG4理论分子量 · 25.8 kDa

产品简介

Recombinant Human IgG4 Fc Protein is expressed in HEK293 cells without tag. It contains amino acid residues Glu99-Gly326 (UniProt accession: P01861).

产品详情

分子别名: IGHG4
蛋白长度: Glu99-Gly326
表达系统: HEK293
理论分子量: The protein has a predicted MW of 25.8 kDa. Due to glycosylation, the protein migrates to 30-35 kDa based on Bis-Tris PAGE result.
纯度: > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
内毒素: Less than 0.1 EU per μg by the LAL method.
缓冲液 / 制剂: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
状态: 冻干粉
储存条件: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
复溶建议: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

数据展示

Bis-Tris PAGE Data — Human IgG4 Fc on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Human IgG4 Fc is greater than 90% as determined by SEC-HPLC.

SPR Data — Human FcRn, His Tag (Cat. FRN-HM101) captured on CM5 Chip via Anti-His Antibody can bind Human IgG4 Fc, No Tag with an affinity constant of 0.10 μM as determined in SPR assay (Biacore T200).

BLI Data — Loaded Human FcRn, His Tag (Cat. FRN-HM101) on Anti-His-Biosensor can bind Human IgG4 Fc, No Tag with an affinity constant of 30.30 nM as determined in BLI assay. (QC Test)

背景介绍

It is known as a IgG4-related disease and its differentiation is based on the analysis of IgG4 levels in the affected tissues. The IgG4-related disease is considered to be a generalized pathological process involving a wide spectrum of various disorders that may affect distant organs.

文献引用

1.Touzani F, Pozdzik A. New insights into immune cells cross-talk during IgG4-related disease. Clin Immunol. 2019;198:1-10. doi:10.1016/j.clim.2018.11.004

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Human IgG4 Fc Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

SPR

SPR Data

BLI

BLI Data

背景介绍

文献引用

Human IgG4 Fc Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

SPR

SPR Data

BLI

BLI Data

背景介绍

文献引用