Human IgG1 Fc Protein, Ultra Low Endotoxin

Name: Human IgG1 Fc Protein, Ultra Low Endotoxin
Brand: 普维拓生物
SKU: IGG1FC-HP002

货号IGG1FC-HP002

HumanHEK293分子名 · IgG1理论分子量 · 25.5 kDa

产品简介

Recombinant Human IgG1 Fc is expressed in HEK293 cells without tag. It contains amino acid residues Asp104-Lys330 (UniProt accession: P01857-1).

产品详情

分子别名: IgG1; Igh-4; RGD1359539; VH7183
蛋白长度: Asp104-Lys330
表达系统: HEK293
理论分子量: The protein has a predicted MW of 25.5 kDa. Due to glycosylation, the protein migrates to 31-33 kDa based on Bis-Tris PAGE result.
纯度: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
内毒素: Less than 0.01 EU per μg by the LAL method.
缓冲液 / 制剂: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
状态: 冻干粉
储存条件: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
复溶建议: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

数据展示

Bis-Tris PAGE Data — Human IgG1 Fc on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Human IgG1 Fc is greater than 95% as determined by SEC-HPLC.

SPR Data — Human FcRn, His Tag captured on CM5 Chip via anti-his antibody can bind Human IgG1 Fc, No Tag with an affinity constant of 0.28 μM as determined in SPR assay (Biacore T200).

BLI Data — Loaded Biotinylated Human FcRn, His-Avi tag on Streptavidin-Biosensor can bind Human IgG1 Fc, No Tag with an affinity constant of 22.8 nM as determined in BLI assay. (QC Test)

背景介绍

IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors (FcγR) on phagocytic cells with high affinity. Fc fragment is demonstrated to mediate phagocytosis, trigger inflammation, and target Ig to particular tissues.IgG1 Fc was reported has a novel role as a potential anti-inflammatory drug for treatment of human autoimmune diseases.

文献引用

1.Ponstingl H, Hilschmann N. [Rule of antibody structure. The primary structure of a monoclonal IgG1 immunoglobulin (myeloma protein Nie), II: isolation and amino acid sequence of the tyrptic peptides from the H-chain (author's transl)].[J]. Hoppe Seylers Zeitschrift Für Physiologische Chemie, 1976, 357(11):1541.

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Human IgG1 Fc Protein, Ultra Low Endotoxin

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

SPR

SPR Data

BLI

BLI Data

背景介绍

文献引用

Human IgG1 Fc Protein, Ultra Low Endotoxin

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

SPR

SPR Data

BLI

BLI Data

背景介绍

文献引用