Human HLA-A*03:01&B2M&GP100 (ALLAVGATK) Tetramer Protein

Name: Human HLA-A*03:01&B2M&GP100 (ALLAVGATK) Tetramer Protein
Brand: 普维拓生物
SKU: GP100-HP004

货号GP100-HP004

HumanHEK293分子名 · gp100 (ALLAVGATK)理论分子量 · 258 kDa

产品简介

Recombinant Human HLA-A*03:01&B2M&gp100 (ALLAVGATK) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus, tetramer is assembled by biotinylated monomer and streptavidin. It contains Gly25-Thr305 (HLA-A*03:01), Ile21-Met119 (B2M) and ALLAVGATK peptide (accession: NP_002107.3(HLA-A*03:01) & P61769(B2M) &ALLAVGATK).

产品详情

分子别名: gp100 (ALLAVGATK)
蛋白长度: Gly25-Thr305
表达系统: HEK293
理论分子量: The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reduced (N) condition based on Bis-Tris PAGE result.
纯度: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
内毒素: Less than 1 EU per μg by the LAL method.
缓冲液 / 制剂: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
状态: 冻干粉
储存条件: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
复溶建议: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

数据展示

Bis-Tris PAGE Data — Human HLA-A*03:01&B2M&gp100 (ALLAVGATK) Tetramer on Bis-Tris PAGE under Non reduced (N) condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Human HLA-A*03:01&B2M&gp100 (ALLAVGATK) Tetramer is greater than 95% as determined by SEC-HPLC.

背景介绍

Immunization against the ALLAVGATK peptide may occur in melanoma patients and that the immunogenicity of this peptide is similar to that of the majority of melanoma differentiation antigens defined to date. Therefore, the use of ALLAVGATK in active immunotherapy for HLA-A3 melanoma patients, for whom no other immunogenic peptide has been so far described, remains a distinct possibility, providing that patients treated in vivo with this peptide develop a better in vitro response than already demonstrated for HLA-A2 epitopes generated from this same antigenic protein.

文献引用

1.Castelli C, Mazzocchi A, Rini F, Tarsini P, Rivoltini L, Maio M, Gallino G, Belli F, Parmiani G. Immunogenicity of the ALLAVGATK (gp100[17-25]) peptide in HLA-A3.1 melanoma patients. Eur J Immunol. 1998 Apr;28(4):1143-54. doi: 10.1002/(SICI)1521-4141(199804)28:04<1143::AID-IMMU1143>3.0.CO;2-K. PMID: 9565353.

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Human HLA-A*03:01&B2M&GP100 (ALLAVGATK) Tetramer Protein

货号GP100-HP004

HumanHEK293分子名 · gp100 (ALLAVGATK)理论分子量 · 258 kDa

Human HLA-A*03:01&B2M&GP100 (ALLAVGATK) Tetramer Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

背景介绍

文献引用

Human HLA-A*03:01&B2M&GP100 (ALLAVGATK) Tetramer Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

背景介绍

文献引用