Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer Protein

Name: Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer Protein
Brand: 普维拓生物
SKU: GP100-HB001

货号GP100-HB001

HumanHEK293分子名 · HLA0201理论分子量 · 50.4 kDa

产品简介

Recombinant Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and YLEPGPVTA peptide (accession: A0A140T913(HLA-A*02:01) & P61769(B2M) &YLEPGPVTA).

产品详情

分子别名: HLA0201; MHC I; gp100; Glycoprotein 100; ME20-M; P100
蛋白长度: Gly25-Thr305
表达系统: HEK293
理论分子量: The protein has a predicted MW of 50.4 kDa. Due to glycosylation, the protein migrates to 52-62 kDa based on Bis-Tris PAGE result.
纯度: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
内毒素: Less than 1 EU per μg by the LAL method.
缓冲液 / 制剂: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
状态: 冻干粉
储存条件: -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
复溶建议: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

数据展示

Bis-Tris PAGE Data — Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer was greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Antibody, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA (QC Test).

背景介绍

Glycoprotein 100, gp100 or Melanocyte protein PMEL is 661 amino acids long and is a type I transmembrane glycoprotein enriched in melanosomes, which are the melanin-producing organelles in melanocytes. This protein is involved in melanosome maturation. The Human HLA-A*0201 gp100 (YLEPGPVTA) complex Protein is a complex of HLA-A*0201 of the MHC Class I and YLEPGPVTA peptide of the gp100.

文献引用

1.(1)Abad J D, Wrzensinski C, Overwijk W, et al. T-Cell Receptor Gene Therapy of Established Tumors in a Murine Melanoma Model[J]. Journal of Immunotherapy, 2008, 31(1):1-6.

加载中…

Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer Protein

货号GP100-HB001

HumanHEK293分子名 · HLA0201理论分子量 · 50.4 kDa

Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

ELISA

ELISA Data

背景介绍

文献引用

Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

ELISA

ELISA Data

背景介绍

文献引用