Biotinylated Human ENPP-3/CD203c Protein

Name: Biotinylated Human ENPP-3/CD203c Protein
Brand: 普维拓生物
SKU: ENPP3-HB001

货号ENPP3-HB001

HumanHEK293分子名 · E-NPP 3理论分子量 · 99.97 kDa

产品简介

Recombinant Biotinylated Human ENPP-3 Protein is expressed in HEK293 cells with a His tag and Avi tag at the N-terminus. It contains amino acid residues Leu48-Ile875 (UniProt accession: O14638).

产品详情

分子别名: E-NPP 3; NPP3; PD-Ibeta; NPPase; ENPP3; PDNP3; CD203c; B10; gp130RB13-6; PD-Iβ; PD-Ib; PD-I beta
蛋白长度: Leu48-Ile875
表达系统: HEK293
理论分子量: The protein has a predicted MW of 99.97 kDa. Due to glycosylation, the protein migrates to 110-130 kDa based on Bis-Tris PAGE result.
纯度: > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
内毒素: Less than 1 EU per μg by the LAL method.
缓冲液 / 制剂: Lyophilized from 0.22 μm filtered solution in 50mM Tris, 150mM NaCl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
状态: 冻干粉
储存条件: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
复溶建议: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

数据展示

Bis-Tris PAGE Data — Biotinylated Human ENPP-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Human ENPP-3 is greater than 90% as determined by SEC-HPLC.

ELISA Data — Immobilized Biotinylated Human ENPP-3, His Avi tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-ENPP-3 Antibody, hFc Tag with the EC50 of 13.3ng/ml determined by ELISA. (QC Test)

背景介绍

Ectonucleotide pyrophosphatase-phosphodiesterase 3 (ENPP3), a protein detected in the human uterus, has been found to play an important role in the development and invasion of tumours. It was recently discovered that ENPP3 was upregulated during the window of implantation in the human endometrium but its functional relevance remains elusive.

文献引用

1.Chen Q, Xin A, Qu R, Zhang W, Li L, Chen J, Lu X, Gu Y, Li J, Sun X. Expression of ENPP3 in human cyclic endometrium: a novel molecule involved in embryo implantation. Reprod Fertil Dev. 2018 Oct;30(10):1277-1285. doi: 10.1071/RD17257. PMID: 29614240.

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Biotinylated Human ENPP-3/CD203c Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

背景介绍

文献引用

Biotinylated Human ENPP-3/CD203c Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

背景介绍

文献引用