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Recombinant Human CD8 beta/CD8B Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Leu22-Pro170 (UniProt accession: P10966-1).
CD8 on thymocytes and thymus-derived T cells consists of disulfide-linked CD8a and CD8b chains, which are transmembrane proteins, containing N-terminal Ig domains, extended and glycosylated hinge and stalk regions, and transmembrane and cytoplasmic portions. The cytoplasmic portion of CD8β, mainly due to its palmitoylation, mediates partitioning of CD8 in lipid rafts, where it efficiently associates with p56 lck. In addition, the cytoplasmic portion of CD8β mediates constitutive association of CD8 with TCR/CD3. The resulting TCR-CD8 adducts exhibit high affinity for major histocom-patibility complex (MHC)-peptide.
