Biotinylated Human Transferrin R/CD71 Protein

Name: Biotinylated Human Transferrin R/CD71 Protein
Brand: 普维拓生物
SKU: CD71-HB001

货号CD71-HB001

HumanHEK293分子名 · TR理论分子量 · 77.9 kDa

产品简介

Recombinant Biotinylated Human Transferrin R/CD71 Protein is expressed in HEK293 cells with a His tag and Avi tag at the N-terminus. It contains amino acid residues Cys89-Phe760 (UniProt accession: P02786).

产品详情

分子别名: TR; TfR1; CD71; sTfR; T9; p90; TFRC; TFR
蛋白长度: Cys89-Phe760
表达系统: HEK293
理论分子量: The protein has a predicted MW of 77.9 kDa. Due to glycosylation, the protein migrates to 78-95 kDa based on Bis-Tris PAGE result.
纯度: > 95% as determined by Bis-Tris PAGE > 85% as determined by HPLC
内毒素: Less than 1 EU per μg by the LAL method.
缓冲液 / 制剂: Lyophilized from 0.22μm filtered solution in PBS, 200mM L-arginine, 8% trehalose (pH 7.4).
状态: 冻干粉
储存条件: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
复溶建议: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

数据展示

Bis-Tris PAGE Data — Biotinylated Human Transferrin R on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Human Transferrin R is greater than 85% as determined by SEC-HPLC.

SEC-MALS Data — The purity of Biotinylated Human Transferrin R is greater than 85% and the molecular weight of this protein is around 160-180 kDa as determined by SEC-MALS.

ELISA Data — Immobilized Human Transferrin, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Transferrin R, His Tag with the EC50 of 66.4ng/ml determined by ELISA (QC Test).

SPR Data — Human Transferrin, His Tag immobilized on CM5 Chip can bind Biotinylated Human Transferrin R, His-Avi tag with an affinity constant of 7.73 nM as determined in SPR assay (Biacore T200).

背景介绍

The transferrin receptor (CD71) is known as a receptor for IgA1 on mesangial cells. The transcript and protein expression of glomerular CD71 was determined by real-time polymerase chain reaction and immunohistochemistry. Human mesangial cells (HMCs) were treated with sera from IgAN patients and expression levels of CD71 and inflammatory cytokine markers were compared according to disease status.

文献引用

1.Jhee JH, Nam BY, Park JT, Kim HW, Chang TI, Kang EW, Lim BJ, Yoo TH, Kang SW, Jeong HJ, Han SH. CD71 mesangial IgA1 receptor and the progression of IgA nephropathy. Transl Res. 2021 Apr;230:34-43. doi: 10.1016/j.trsl.2020.10.007. Epub 2020 Oct 24. PMID: 33122053.

加载中…

Biotinylated Human Transferrin R/CD71 Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

SEC-MALS

SEC-MALS Data

ELISA

ELISA Data

ELISA

ELISA Data

SPR

SPR Data

背景介绍

文献引用

Biotinylated Human Transferrin R/CD71 Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

SEC-MALS

SEC-MALS Data

ELISA

ELISA Data

ELISA

ELISA Data

SPR

SPR Data

背景介绍

文献引用