Non-biotinylated Human Fc gamma RI/CD64 Protein

Name: Non-biotinylated Human Fc gamma RI/CD64 Protein
Brand: 普维拓生物
SKU: CD64-HP001

货号CD64-HP001

HumanHEK293分子名 · CD64理论分子量 · 33.5 kDa

产品简介

Recombinant Human Fc gamma RI/CD64 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Gln16-Pro288 (UniProt accession: P12314-1).

产品详情

分子别名: CD64; CD64a; Fc gamma RI; FCG1; Fc-gamma RIA; FCGR1; FcgRI; FCRI; FcRIA; FLJ18345; FCGR1A; IGFR1; Fcr; Fcgr; FCE1A; FcERI; FcγRI; Fc γRI; Fcγ RI; Fc γ RI
蛋白长度: Gln16-Pro288
表达系统: HEK293
理论分子量: The protein has a predicted MW of 33.5 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
纯度: > 95% as determined by Bis-Tris PAGE
内毒素: Less than 1 EU per μg by the LAL method.
缓冲液 / 制剂: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
状态: 冻干粉
储存条件: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
复溶建议: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

数据展示

Bis-Tris PAGE Data — Human Fc gamma RI on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SPR Data — Human Fc gamma RI, His Tag captured on CM5 Chip via anti-his antibody can bind Trastuzumab with an affinity constant of 5.25 nM as determined in SPR assay (Biacore T200). (QC Test)

BLI Data — Loaded Human Fc gamma RI, His-Avi tag on Anti-His-Biosensor, can bind Trastuzumab with an affinity constant of 11.70 nM as determined in BLI assay (Gator).

背景介绍

The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type.

文献引用

1.Nimmerjahn F, Ravetch J V. Fcγ receptors as regulators of immune responses[J]. Nature Reviews Immunology, 2008, 8(1):34-47.

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Non-biotinylated Human Fc gamma RI/CD64 Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SPR

SPR Data

BLI

BLI Data

背景介绍

文献引用

Non-biotinylated Human Fc gamma RI/CD64 Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SPR

SPR Data

BLI

BLI Data

背景介绍

文献引用