Biotinylated Human Siglec-3/CD33 Protein

Name: Biotinylated Human Siglec-3/CD33 Protein
Brand: 普维拓生物
SKU: CD33-HB001

货号CD33-HB001

HumanHEK293分子名 · CD33 molecule理论分子量 · 29.6 kDa

产品简介

Recombinant Biotinylated Human Siglec-3/CD33 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Asp18-His259 (UniProt accession: P20138-1).

产品详情

分子别名: CD33 molecule; CD33; FLJ00391; gp67; Siglec3; Siglec-3; p67
蛋白长度: Asp18-His259
表达系统: HEK293
理论分子量: The protein has a predicted MW of 29.6 kDa. Due to glycosylation, the protein migrates to 48-58 kDa based on Bis-Tris PAGE result.
纯度: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
内毒素: Less than 1 EU per μg by the LAL method.
缓冲液 / 制剂: Supplied as 0.22μm filtered solution in 50mM MES, 500mM NaCl, 200mM L-arginine (pH 7.4).
储存条件: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

数据展示

Bis-Tris PAGE Data — Biotinylated Human Siglec-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Human Siglec-3 is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Biotinylated Human Siglec-3, His Tag at 0.5μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Anti-Siglec-3 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA.

背景介绍

CD33 (siglec-3), a well-known target in leukemia therapy, is an inhibitory sialoadhesin expressed in human leukocytes of the myeloid lineage and some lymphoid subsets, including NK cells. CD33 is the smallest siglec member. It preferentially binds to α2-6- and α2-3-sialylated glycans and strongly binds to sialylated ligands on leukemic cell lines.

文献引用

1.Hernández-Caselles T, Miguel RC, Ruiz-Alcaraz AJ, García-Peñarrubia P. CD33 (Siglec-3) Inhibitory Function: Role in the NKG2D/DAP10 Activating Pathway. J Immunol Res. 2019 Apr 15;2019:6032141. doi: 10.1155/2019/6032141. PMID: 31143782; PMCID: PMC6501159.

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Biotinylated Human Siglec-3/CD33 Protein

货号CD33-HB001

HumanHEK293分子名 · CD33 molecule理论分子量 · 29.6 kDa

Biotinylated Human Siglec-3/CD33 Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

背景介绍

文献引用

Biotinylated Human Siglec-3/CD33 Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

背景介绍

文献引用