Human BAFF/TNFSF13B/CD257 Protein (active trimer)

Name: Human BAFF/TNFSF13B/CD257 Protein (active trimer)
Brand: 普维拓生物
SKU: CD257-HF002

货号CD257-HF002

HumanHEK293分子名 · BAFF理论分子量 · 41.7 kDa

产品简介

Recombinant Human BAFF/TNFSF13B/CD257 Protein is expressed in HEK293 cells with monomeric Fc tag at the N-terminus. It contains amino acid residues Ala134-Leu285 (UniProt accession: Q9Y275-1).

产品详情

分子别名: BAFF; BLyS; CD257; TNFSF13B; TNFSF20; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; ZTNF4; TALL-1;TALL1delta BAFF; TALL1Delta4BAFF
蛋白长度: Ala134-Leu285
表达系统: HEK293
理论分子量: The protein has a predicted MW of 41.7 kDa. Due to glycosylation, the protein migrates to 45-52 kDa based on Bis-Tris PAGE result.
纯度: > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
内毒素: Less than 1 EU per μg by the LAL method.
缓冲液 / 制剂: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
状态: 冻干粉
储存条件: -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
复溶建议: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

数据展示

Bis-Tris PAGE Data — Human BAFF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Human BAFF is greater than 90% as determined by SEC-HPLC.

SEC-MALS Data — The purity of Human BAFF is greater than 90% and the molecular weight of this protein is around 125-150 kDa as determined by SEC-MALS.

ELISA Data — Immobilized Human BCMA Trimer, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human BAFF, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA (QC Test).

SPR Data — Human BAFF, hFc Tag captured on CM5 Chip via Protein A can bind Mouse BCMA, His-Avi tag with an affinity constant of 5.83 μM as determined in SPR assay (Biacore T200).

BLI Data — Loaded Human BAFF, monomeric hFc Tag on ProA-Biosensor can bind Human BCMA (Trimer), His Tag with an affinity constant of 0.36 nM as determined in BLI assay.

背景介绍

B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R.

文献引用

1.Bossen C, Schneider P. BAFF, APRIL and their receptors: Structure, function and signaling[J]. Seminars in Immunology, 2006, 18(5):0-275. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.

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Human BAFF/TNFSF13B/CD257 Protein (active trimer)

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

SEC-MALS

SEC-MALS Data

ELISA

ELISA Data

ELISA

ELISA Data

ELISA

ELISA Data

SPR

SPR Data

BLI

BLI Data

BLI

BLI Data

背景介绍

文献引用

Human BAFF/TNFSF13B/CD257 Protein (active trimer)

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

SEC-MALS

SEC-MALS Data

ELISA

ELISA Data

ELISA

ELISA Data

ELISA

ELISA Data

SPR

SPR Data

BLI

BLI Data

BLI

BLI Data

背景介绍

文献引用