Human IL-17R alpha/CD217 Protein

Name: Human IL-17R alpha/CD217 Protein
Brand: 普维拓生物
SKU: CD217-HF001

货号CD217-HF001

HumanHEK293分子名 · CD217理论分子量 · 60.2 kDa

产品简介

Recombinant Human IL-17R alpha/CD217 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Leu33-Trp320 (UniProt accession: Q96F46-1).

产品详情

分子别名: CD217; CDw217; IL-17RA; IL17R; CANDF5; IL-17R; IL-17R-alpha; IL17Rα;IL17RA; IL17R-alpha;IL17R alpha
蛋白长度: Leu33-Trp320
表达系统: HEK293
理论分子量: The protein has a predicted MW of 60.2 kDa. Due to glycosylation, the protein migrates to 75-90 kDa based on Bis-Tris PAGE result.
纯度: > 95% as determined by Bis-Tris PAGE
内毒素: Less than 1 EU per ug by the LAL method.
缓冲液 / 制剂: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
状态: 冻干粉
储存条件: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
复溶建议: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

数据展示

Bis-Tris PAGE Data — Human IL-17R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

ELISA Data — Immobilized Human IL-17A, His Tag (Cat. ILA-HM118) at 1μg/ml (100μl/well) on the plate. Dose response curve for Human IL-17R alpha, hFc Tag with the EC50 of 72.2ng/ml determined by ELISA. (QC Test)

SPR Data — Human IL-17R alpha, hFc Tag captured on CM5 Chip via protein A can bind Biotinylated Human IL-17A, His-Avi tag (Cat. ILA-HM418B) with an affinity constant of 2.18 nM as determined in SPR assay (Biacore T200).

BLI Data — Loaded Human IL-17A, His Tag on Anti-His-Biosensor can bind Human IL-17R alpha, hFc Tag with an affinity constant of 9.85 nM as determined in BLI assay.

背景介绍

Interleukin 17 (also known as CTLA‑8) is a T cell‑expressed pleotropic cytokine. IL‑17 binds to IL‑17 receptor (IL‑17 R) which shares no homology with any known family of receptors. While the expression of IL‑17 is restricted to activated T cells, the IL‑17 R mRNA exhibits a broad tissue distribution, and has been detected in virtually all cells and tissues tested. The human IL‑17 R gene was localized to chromosome 22.

文献引用

1.Gaffen S L. An overview of IL-17 function and signaling[J]. Cytokine, 2008, 43(3):0-407.

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Human IL-17R alpha/CD217 Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

ELISA

ELISA Data

ELISA

ELISA Data

ELISA

ELISA Data

SPR

SPR Data

BLI

BLI Data

背景介绍

文献引用

Human IL-17R alpha/CD217 Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

ELISA

ELISA Data

ELISA

ELISA Data

ELISA

ELISA Data

SPR

SPR Data

BLI

BLI Data

背景介绍

文献引用