Biotinylated Human HLA-A*02:01&B2M&AFP (PLFQVPEPV) Monomer Protein

Name: Biotinylated Human HLA-A*02:01&B2M&AFP (PLFQVPEPV) Monomer Protein
Brand: 普维拓生物
SKU: AFP-HB002

货号AFP-HB002

HumanHEK293分子名 · Alpha-fetoprotein理论分子量 · 50.4 kDa

产品简介

Recombinant Biotinylated Human HLA-A*02:01&B2M&AFP (PLFQVPEPV) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and PLFQVPEPV peptide (accession: A0A140T913(HLA-A*02:01) & P61769(B2M) &PLFQVPEPV).

产品详情

分子别名: Alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-feto; AFP; HPAFP; AFPD; FETA; MHC
蛋白长度: Gly25-Thr305
表达系统: HEK293
理论分子量: The protein has a predicted MW of 50.4 kDa. Due to glycosylation, the protein migrates to 53-63 kDa based on Bis-Tris PAGE result.
纯度: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
内毒素: Less than 1 EU per μg by the LAL method.
缓冲液 / 制剂: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
状态: 冻干粉
储存条件: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
复溶建议: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

数据展示

Bis-Tris PAGE Data — Biotinylated Human HLA-A*02:01&B2M&AFP (PLFQVPEPV) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Human HLA-A*02:01&B2M&AFP (PLFQVPEPV) Monomer is greater than 95% as determined by SEC-HPLC.

背景介绍

Alpha-fetoprotein (AFP), a specific liver cancer marker, T cells expressing AFP-CAR selectively degranulated, released cytokines, and lysed liver cancer cells that were HLA-A*02:01 /AFP while sparing cells from multiple tissue types that were negative for either expressed proteins.CAR T-cell immunotherapy targeting intracellular/secreted solid tumor antigens can elicit a potent antitumor response.

文献引用

1.(1) Liu H, Xu Y, Xiang J, Long L, Green S, Yang Z, Zimdahl B, Lu J, Cheng N, Horan LH, Liu B, Yan S, Wang P, Diaz J, Jin L, Nakano Y, Morales JF, Zhang P, Liu LX, Staley BK, Priceman SJ, Brown CE, Forman SJ, Chan VW, Liu C. Targeting Alpha-Fetoprotein (AFP)-MHC Complex with CAR T-Cell Therapy for Liver Cancer. Clin Cancer Res. 2017 Jan 15;23(2):478-488. doi: 10.1158/1078-0432.CCR-16-1203. Epub 2016 Aug 17. PMID: 27535982.

加载中…

Biotinylated Human HLA-A*02:01&B2M&AFP (PLFQVPEPV) Monomer Protein

货号AFP-HB002

HumanHEK293分子名 · Alpha-fetoprotein理论分子量 · 50.4 kDa

Biotinylated Human HLA-A*02:01&B2M&AFP (PLFQVPEPV) Monomer Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

背景介绍

文献引用

Biotinylated Human HLA-A*02:01&B2M&AFP (PLFQVPEPV) Monomer Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

背景介绍

文献引用