Biotinylated Human HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Monomer Protein

Name: Biotinylated Human HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Monomer Protein
Brand: Puweituo Bio
SKU: PRAME-HB002

Catalog numberPRAME-HB002

HumanHEK293Molecule · PRAMEMolecular weight · 50.60 kDa

Product introduction

Recombinant Biotinylated Human HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and ALYVDSLFFL peptide (accession: P79603(HLA-A*02:01) & P61769(B2M) &ALYVDSLFFL).

Product details

Molecular aliases: PRAME; OIP-4; OIP4; MAPE;
Protein length: Gly25-Thr305
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 50.60 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22 μm filtered solution in PBS, 100mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Biotinylated Human HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Human HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Monomer is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Biotinylated Human HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Monomer, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Antibody, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA.

Background

PRAME (PReferentially expressed Antigen in MElanoma) is a melanoma-associated antigen expressed in cutaneous and ocular melanomas and some other malignant neoplasms, while its expression in normal tissue and benign tumors is limited.

References

1.Lezcano C, Jungbluth AA, Busam KJ. PRAME Immunohistochemistry as an Ancillary Test for the Assessment of Melanocytic Lesions. Surg Pathol Clin. 2021 Jun;14(2):165-175. doi: 10.1016/j.path.2021.01.001. Epub 2021 Apr 28. PMID: 34023098; PMCID: PMC8152939.

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Biotinylated Human HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Monomer Protein

Catalog numberPRAME-HB002

HumanHEK293Molecule · PRAMEMolecular weight · 50.60 kDa

Biotinylated Human HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Monomer Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References

Biotinylated Human HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Monomer Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References