Biotinylated Rat MASP2 Protein (Primary Amine Labeling)

Name: Biotinylated Rat MASP2 Protein (Primary Amine Labeling)
Brand: Puweituo Bio
SKU: MASP2-RB001

Catalog numberMASP2-RB001

RatE. coliMolecule · MAP19Molecular weight · 46.7 kDa

Product introduction

Recombinant Biotinylated Rat MASP2 Protein (Primary Amine Labeling) is expressed in E. coli with a His tag at the C-terminus. It contains amino acid residues Thr287-Phe685 (accession: A2VCV7).

Product details

Molecular aliases: MAP19; MASP-2; MASP1P1; sMap;MASP2
Protein length: Thr287-Phe685
Expression system: E. coli
Molecular weight: The protein has a predicted MW of 46.7 kDa. Due to autocatalytic cleavage, the protein migrates to 15-20 kDa&25-30 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Supplied as 0.22μm filtered solution in 50mM Tris, 200mM NaCl (pH 9.0).
Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Data display

Bis-Tris PAGE Data — Biotinylated Rat MASP2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

ELISA Data — Immobilized Narsoplimab, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Rat MASP2, His Tag with the EC50 of 19.3ng/ml determined by ELISA. (QC Test)

Background

Te MASP2 gene is located on the chromosome 1p36.23–31 and encodes two proteins, MASP-2 and Map198. Both the levels and function of MASP-2 are modulated by the single-nucleotide polymorphisms (SNPs) of the MASP2 gene. Te level of MASP-2 in plasma plays a dual role in the infection and progression of infectious diseases. Te defciency of MASP-2 in plasma has been associated with the susceptibility of HIV. By contrast, the overproduction of MASP-2 may increase the infammatory efect.

References

1.Li Z, Wang M, Zhong H, Huang X, Wu X, Zhang X, Wang J, Deng J, Chen M, Chen L, Tan H. Impact of MASP2 gene polymorphism and gene-tea drinking interaction on susceptibility to tuberculosis. Sci Rep. 2021 Mar 22;11(1):6544. doi: 10.1038/s41598-021-86129-x. PMID: 33753877; PMCID: PMC7985323.

加载中…

Biotinylated Rat MASP2 Protein (Primary Amine Labeling)

Catalog numberMASP2-RB001

RatE. coliMolecule · MAP19Molecular weight · 46.7 kDa

Product introduction

Recombinant Biotinylated Rat MASP2 Protein (Primary Amine Labeling) is expressed in E. coli with a His tag at the C-terminus. It contains amino acid residues Thr287-Phe685 (accession: A2VCV7).

Biotinylated Rat MASP2 Protein (Primary Amine Labeling)

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

ELISA

ELISA Data

Background

References

Biotinylated Rat MASP2 Protein (Primary Amine Labeling)

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

ELISA

ELISA Data

Background

References