Human IL-15 Protein

Name: Human IL-15 Protein
Brand: Puweituo Bio
SKU: IL15-HP001

Catalog numberIL15-HP001

HumanE. coliMolecule · L15Molecular weight · 12.8 kDa

Product introduction

Recombinant Human IL-15 Protein is expressed in E. coli without tag. It contains amino acid residues Asn49-Ser162 (UniProt accession: P40933-1).

Product details

Molecular aliases: L15; IL-15; IL-15MGC9721; Interleukin-15
Protein length: Asn49-Ser162
Expression system: E. coli
Molecular weight: The protein has a predicted MW of 12.8 kDa same as Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 0.05 EU per μg by the LAL method.
Formulation: Supplied as 0.22 μm filtered solution in 20mM Tris, 150mM NACI (pH 7.5).
Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Data display

Bis-Tris PAGE Data — Human IL-15 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Human IL-15 is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Human IL-15, No Tag at 2 μg/ml (100 μl/Well) on the plate. Dose response curve for Human IL-15RA, His Tag with the EC50 of 15.4 ng/ml determined by ELISA.

SPR Data — Human IL-2 R beta, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-15, No Tag with an affinity constant of 5.9 nM as determined in SPR assay (Biacore T200).

Cell Proliferation Bioactivity — Measured in a cell proliferation assay using CTLL-2 cells. The ED50 for this effect is 0.1 - 0.3 ng/mL. The specific activity of recombinant human IL-15 is > 1 × 10^7 units/mg, which is calibrated against the human IL-15 reference standard (NIBSC code: 95/554) (QC Test).

Background

Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 and plays an important role in many immunological diseases. IL-15 is a cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL15 requires interaction of IL15 with components of the IL2 receptor, including IL2RB and probably IL2RG but not IL2RA.

References

1.Rautela J, Huntington N D. IL-15 signaling in NK cell cancer immunotherapy [J]. Current Opinion in Immunology, 2017, 44:1-6.

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Human IL-15 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

SPR

SPR Data

Bioactivity

Cell Proliferation Bioactivity

Background

References

Human IL-15 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

SPR

SPR Data

Bioactivity

Cell Proliferation Bioactivity

Background

References