Biotinylated Human HLA-A*02:01&B2M&Influenza M1₅₈₋₆₆ (GILGFVFTL) Monomer Protein

Name: Biotinylated Human HLA-A*02:01&B2M&Influenza M1₅₈₋₆₆ (GILGFVFTL) Monomer Protein
Brand: Puweituo Bio
SKU: HLAA0201-HB008

Catalog numberHLAA0201-HB008

HumanE. coliMolecule · IAV M1 (GILGFVFTL)Molecular weight · 35.6 kDa

Product introduction

Recombinant Biotinylated Human HLA-A*02:01&B2M&Influenza M1₅₈₋₆₆ (GILGFVFTL) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and GILGFVFTL peptide (accession: A0A140T913(HLA-A*02:01) & P61769(B2M) &GILGFVFTL).

Product details

Molecular aliases: IAV M1 (GILGFVFTL); IAV M1; GILGFVFTL
Protein length: Gly25-Thr305
Expression system: E. coli
Molecular weight: The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Supplied as 0.22 μm filtered solution in 20mM Tris, 200mM NaCl (pH 8.0).
Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Data display

Bis-Tris PAGE Data — Biotinylated Human HLA-A*02:01&B2M&Influenza M1₅₈₋₆₆ (GILGFVFTL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Human HLA-A*02:01&B2M&Influenza M1₅₈₋₆₆ (GILGFVFTL) Monomer is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Biotinylated Human HLA-A*02:01&B2M&Influenza M158-66 (GILGFVFTL) Monomer, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 19.8ng/ml determined by ELISA..

Background

Influenza A virus (IAV) is a respiratory pathogen that causes seasonal epidemics with significant mortality. One of the most abundant proteins in IAV particles is the matrix protein 1 (M1), which is essential for the virus structural stability. M1 organizes virion assembly and budding at the plasma membrane (PM), where it interacts with other viral components.

References

1.Petrich A, Dunsing V, Bobone S, Chiantia S. Influenza A M2 recruits M1 to the plasma membrane: A fluorescence fluctuation microscopy study. Biophys J. 2021 Dec 21;120(24):5478-5490. doi: 10.1016/j.bpj.2021.11.023. Epub 2021 Nov 19. PMID: 34808098; PMCID: PMC8715234.

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Biotinylated Human HLA-A*02:01&B2M&Influenza M1₅₈₋₆₆ (GILGFVFTL) Monomer Protein

Catalog numberHLAA0201-HB008

HumanE. coliMolecule · IAV M1 (GILGFVFTL)Molecular weight · 35.6 kDa

Biotinylated Human HLA-A*02:01&B2M&Influenza M1₅₈₋₆₆ (GILGFVFTL) Monomer Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References

Biotinylated Human HLA-A*02:01&B2M&Influenza M1₅₈₋₆₆ (GILGFVFTL) Monomer Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References