Biotinylated Human Claudin 18.2 Nanodisc

Name: Biotinylated Human Claudin 18.2 Nanodisc
Brand: Puweituo Bio
SKU: CLDN182-HB004

Catalog numberCLDN182-HB004

HumanHEK293Molecule · CLDN18Molecular weight · 40.60 kDa

Product introduction

Recombinant Biotinylated Human Claudin 18.2 Nanodisc is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Met1-Val261 (UniProt accession: P56856-2).

Product details

Molecular aliases: CLDN18; CLDN18.2; Claudin-18.2; Claudin18.2; Claudin-18; DKFZp564B2062; SFTA5; SFTPJ
Protein length: Met1-Val261
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 40.60 kDa.
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Supplied as 0.22 μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.
Storage: Valid for 6 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Negative control: CON-HM1NB

Data display

ELISA Data — Immobilized Biotinylated Human Claudin 18.2 Nanodisc, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-Claudin18.2 Antibody, hFc Tag with the EC50 of 52.2ng/ml determined by ELISA. (QC Test)

SPR Data — Biotinylated Human Claudin 18.2 Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Anti-Claudin18.2 Antibody with an affinity constant of 5.72 nM as determined in SPR assay (Biacore T200).

Background

Claudin18 (CLDN18) belongs to the large claudin family of proteins, which form tight junction strands in epithelial cells. CLDN18 is specifically expressed in the stomach and lung. CLDN18 has two alternatively spliced variants, CLDN18.1 and CLDN18.2. Isoform 2 (Claudin 18.2) is abundant in gastric tumors.

References

1.Singh P, Toom S, Huang Y. Anti-claudin 18.2 antibody as new targeted therapy for advanced gastric cancer[J]. Journal of Hematology & Oncology, 2017, 10(1):105.

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Biotinylated Human Claudin 18.2 Nanodisc

Catalog numberCLDN182-HB004

HumanHEK293Molecule · CLDN18Molecular weight · 40.60 kDa

Product introduction

Recombinant Biotinylated Human Claudin 18.2 Nanodisc is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Met1-Val261 (UniProt accession: P56856-2).

Biotinylated Human Claudin 18.2 Nanodisc

Product introduction

Product details

Data display

ELISA

ELISA Data

SPR

SPR Data

Background

References

Biotinylated Human Claudin 18.2 Nanodisc

Product introduction

Product details

Data display

ELISA

ELISA Data

SPR

SPR Data

Background

References