Cynomolgus CCR8 Protein

Name: Cynomolgus CCR8 Protein
Brand: Puweituo Bio
SKU: CCR8-CF001

Catalog numberCCR8-CF001

HEK293Molecule · CCR-8Molecular weight · 29.73 kDa

Product introduction

Recombinant Cynomolgus CCR8 Protein is expressed in HEK293 cells with an mFc (IgG1) tag at the C-terminus. It contains amino acid residues Met1-Lys35 (accession: XP_015300839.1).

Product details

Molecular aliases: CCR-8; CMKBRL2; CKR-L1; GPR-CY6; GPRCY6; TER1; CCR8; CKRL1; CMKBR8; CD198; ChemR1; CKR-L1; CY6; TER-1; CDw198
Protein length: Met1-Lys35
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 29.73 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Cynomolgus CCR8 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Cynomolgus CCR8 is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Cynomolgus CCR8, mFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-CCR8 Antibody, hFc Tag with the EC50 of 6.1ng/ml determined by ELISA. (QC Test)

SPR Data — Anti-CCR8 Antibody, hFc Tag captured on CM5 Chip via Anti-Human Fc IgG Antibody can bind Cynomolgus CCR8, mFc Tag with an affinity constant of 0.27 μM as determined in SPR assay (Biacore T200).

Background

CC chemokine receptor (CCR) 8 (previously called CKR-L1 or TER1 and designated CD198), which is expressed on Th2 cells and eosinophils, has been implicated in allergic diseases. CCR8 may regulate monocyte chemotaxis and thymic cell line apoptosis and is alternative coreceptor with CD4 for HIV-1 infection.

References

1.Berenguer J, Lagerweij T, Zhao XW, Dusoswa S, van der Stoop P, Westerman B, de Gooijer MC, Zoetemelk M, Zomer A, Crommentuijn MHW, Wedekind LE, López-López À, Giovanazzi A, Bruch-Oms M, van der Meulen-Muileman IH, Reijmers RM, van Kuppevelt TH, García-Vallejo JJ, van Kooyk Y, Tannous BA, Wesseling P, Koppers-Lalic D, Vandertop WP, Noske DP, van Beusechem VW, van Rheenen J, Pegtel DM, van Tellingen O, Wurdinger T. Glycosylated extracellular vesicles released by glioblastoma cells are decorated by CCL18 allowing for cellular uptake via chemokine receptor CCR8. J Extracell Vesicles. 2018 Mar 13;7(1):1446660. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.

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Cynomolgus CCR8 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

SPR

SPR Data

Background

References

Cynomolgus CCR8 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

SPR

SPR Data

Background

References