Biotinylated Human CCR4 Nanodisc

Name: Biotinylated Human CCR4 Nanodisc
Brand: Puweituo Bio
SKU: CCR4-HB001

Catalog numberCCR4-HB001

HumanHEK293Molecule · C-C CKR-4Molecular weight · 54.3 kDa

Product introduction

Recombinant Biotinylated Human CCR4 Nanodisc is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Met1-Leu360 (UniProt accession: P51679).

Product details

Molecular aliases: C-C CKR-4; CC-CKR-4; CCR-4; CCR4; K5-5; CD194; CMKBR4; CKR-4; C-C CKR4; CC-CKR4; CKR4
Protein length: Met1-Leu360
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 54.3 kDa.
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Supplied as 0.22 μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.
Storage: Valid for 6 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Negative control: CON-HM1NB

Data display

ELISA Data — Immobilized Biotinylated Human CCR4 Nanodisc, His Tag at 5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-CCR4 Antibody, hFc Tag with the EC50 of 7.5ng/ml determined by ELISA. (QC Test)

SPR Data — Biotinylated Human CCR4 Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Anti-CCR4 Antibody, hFc Tag with an affinity constant of 0.64 nM as determined in SPR assay (Biacore T200).

Background

CC chemokine receptor 4 (CCR4) is a chemokine receptor mainly expressed by T cells. CCR4 is important in the pathogenesis of many diseases, such as diabetes, multiple sclerosis, asthma, dermatitis, and cancer. This review briefly characterizes CCR4 and its ligands (CCL17, CCL22, and CCL2), and their contributions to immunological and neoplastic diseases.

References

1.Yoshie O. CCR4 as a Therapeutic Target for Cancer Immunotherapy. Cancers (Basel). 2021 Nov 4;13(21):5542. doi: 10.3390/cancers13215542. PMID: 34771703; PMCID: PMC8583476.
2.Bogacka J, Pawlik K, Ciapała K, Ciechanowska A, Mika J. CC Chemokine Receptor 4 (CCR4) as a Possible New Target for Therapy. Int J Mol Sci. 2022 Dec 9;23(24):15638. doi: 10.3390/ijms232415638. PMID: 36555280; PMCID: PMC9779674.

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Biotinylated Human CCR4 Nanodisc

Product introduction

Product details

Data display

ELISA

ELISA Data

SPR

SPR Data

Background

References

Biotinylated Human CCR4 Nanodisc

Product introduction

Product details

Data display

ELISA

ELISA Data

SPR

SPR Data

Background

References