Human/Rhesus macaque/Mouse/Rat/Canine BMP2 Protein

Name: Human/Rhesus macaque/Mouse/Rat/Canine BMP2 Protein
Brand: Puweituo Bio
SKU: BMP2-HP001

Catalog numberBMP2-HP001

HumanE. coliMolecule · BMP-2Molecular weight · 12.90 kDa

Product introduction

Recombinant Human/Rhesus macaque/Mouse/Rat/Canine BMP2 Protein is expressed in E. coli without tag. It contains amino acid residues Gln283-Arg396 (Human/Rhesus macaque) / Gln279-Arg393 (Mouse/Rat) / Gln282-Arg395 (Canine) (accession: P12643(Human) / G7N2Y0(Rhesus macaque) / P21274(Mouse) / P49001(Rat) / A0A8I3PXE9(Canine)).

Product details

Molecular aliases: BMP-2; BMP-2A; BMP2A; BDA2; SSFSC;
Protein length: Gln283-Arg396
Expression system: E. coli
Molecular weight: The protein has a predicted MW of 12.90 kDa same as Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE
Endotoxin: Less than 0.5 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22μm filtered solution in 100mM Acetic Acid (pH 2.8). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in 100mM Acetic Acid (pH 2.8). Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Human/Rhesus macaque/Mouse/Rat/Canine BMP2 on Bis-Tris PAGE under Non reduced (N) condition and reduced condition. The purity is greater than 95%.

Cell Based Assay Data — Measured by its ability to induce Alkaline Phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 100-400 ng/mL. (QC Test)

Background

Bone morphogenetic protein 2 (BMP2), a member of the transforming growth factor-β (TGF-β) super-family, is one of the main chondrogenic growth factors involved in cartilage regeneration. BMP2 is known to induce chondrogenic differentiation in various types of stem cells in vitro. However, BMP2 also induces osteogenic differentiation and endochondral ossification in mesenchymal stem cells (MSCs).

References

1.PMID: 27083447.

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Human/Rhesus macaque/Mouse/Rat/Canine BMP2 Protein

Catalog numberBMP2-HP001

HumanE. coliMolecule · BMP-2Molecular weight · 12.90 kDa

Human/Rhesus macaque/Mouse/Rat/Canine BMP2 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

Bioactivity

Cell Based Assay Data

Background

References

Human/Rhesus macaque/Mouse/Rat/Canine BMP2 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

Bioactivity

Cell Based Assay Data

Background

References