Biotinylated Human TREM2 Protein

Name: Biotinylated Human TREM2 Protein
Brand: Puweituo Bio
SKU: TREM2-HB001

Catalog numberTREM2-HB001

HumanHEK293Molecule · PLOSL2Molecular weight · 20.3 kDa

Product introduction

Recombinant Biotinylated Human TREM2 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues His19-Ser174 (UniProt accession: Q9NZC2-1).

Product details

Molecular aliases: PLOSL2; TREM2; TREM-2; Trem2a; Trem2b; Trem2c
Protein length: His19-Ser174
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 20.3 kDa. Due to glycosylation, the protein migrates to 30-45 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Biotinylated Human TREM2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

ELISA Data — Immobilized Biotinylated Human TREM2, His Tag at 1μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Anti-TREM2 Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA. (QC Test)

Background

TREM-2 (Triggering Receptor Expressed on Myeloid cells-2) is a 35 kDa type I transmembrane member of the TREM family and Ig superfamily. Mature human TREM-2 consists of a 156 amino acid (aa) extracellular domain (ECD) with one V-type Ig-like domain, a 21 aa transmembrane (TM) domain, and a 35 aa cytoplasmic tail. TREM-2 forms a receptor signaling complex with TYROBP which mediates signaling and cell activation following ligand binding (PubMed:10799849). Acts as a receptor for amyloid-beta protein 42, a cleavage product of the amyloid-beta precursor protein APP, and mediates its uptake and degradation by microglia.

References

1.Ford J W, Mcvicar D W. TREM and TREM-like receptors in inflammation and disease[J]. Current Opinion in Immunology, 2009, 21(1):38-46.

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Biotinylated Human TREM2 Protein

Catalog numberTREM2-HB001

HumanHEK293Molecule · PLOSL2Molecular weight · 20.3 kDa

Product introduction

Recombinant Biotinylated Human TREM2 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues His19-Ser174 (UniProt accession: Q9NZC2-1).

Biotinylated Human TREM2 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

ELISA

ELISA Data

Background

References

Biotinylated Human TREM2 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

ELISA

ELISA Data

Background

References