Biotinylated Human 4-1BB Ligand/TNFSF9 Trimer Protein (Primary Amine Labeling)

Name: Biotinylated Human 4-1BB Ligand/TNFSF9 Trimer Protein (Primary Amine Labeling)
Brand: Puweituo Bio
SKU: TNFSF9-HB001

Catalog numberTNFSF9-HB001

HumanHEK293Molecule · 41BB LigandMolecular weight · 84.3 kDa

Product introduction

Recombinant Biotinylated Human 4-1BB Ligand/TNFSF9 Trimer Protein (Primary Amine Labeling) is expressed in HEK293 cells with Flag tag and monomeric hFc tag at the N-terminus. It contains amino acid residues Arg71-Glu254 trimer design (UniProt accession: P41273).

Product details

Molecular aliases: 41BB Ligand; 4-1BB Ligand; 4-1BBL; CD137L; TNFSF9
Protein length: Arg71-Glu254
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 84.3 kDa same as Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22 μm filtered solution in PBS, 8% trehalose (pH 7.4).
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Biotinylated Human 4-1BB Ligand (Trimer) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Human 4-1BB Ligand (Trimer) is greater than 90% as determined by SEC-HPLC.

ELISA Data — Immobilized Human 4-1BB, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human 4-1BB Ligand (Trimer), hFc Tag with the EC50 of 9.0ng/ml determined by ELISA. (QC Test)

BLI Data — Loaded Biotinylated Human 4-1BB Ligand (Trimer), monomeric hFc Tag on Streptavidin-Biosensor can bind Human 4-1BB, hFc Tag with an affinity constant of 2.19 nM as determined in BLI assay.

Background

The 4-1BBL is the high affinity ligand of 4-1BB, also known as CD137L or TNFSF9. 4-1BB ligand (4-1BBL) is an inducible molecule present on several APC types, including B cells, macrophages and DCs.4-1BB:4-1BBL pathway seems to amplify the existing costimulatory signals, even if the engagement of 4-1BB in the presence of a strong TCR signaling can induce IL-2 production in a CD28-independent manner.

References

1.Vinay D S, Kwon B S. 4-1BB (CD137), an inducible costimulatory receptor, as a specific target for cancer therapy[J]. BMB Reports, 2014, 47(3):122-129.

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Biotinylated Human 4-1BB Ligand/TNFSF9 Trimer Protein (Primary Amine Labeling)

Catalog numberTNFSF9-HB001

HumanHEK293Molecule · 41BB LigandMolecular weight · 84.3 kDa

Biotinylated Human 4-1BB Ligand/TNFSF9 Trimer Protein (Primary Amine Labeling)

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

BLI

BLI Data

Background

References

Biotinylated Human 4-1BB Ligand/TNFSF9 Trimer Protein (Primary Amine Labeling)

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

BLI

BLI Data

Background

References