Biotinylated Cynomolgus SOST/Sclerostin Protein

Name: Biotinylated Cynomolgus SOST/Sclerostin Protein
Brand: Puweituo Bio
SKU: SOST-CB001

Catalog numberSOST-CB001

HEK293Molecule · sclerostinMolecular weight · 24.39 kDa

Product introduction

Recombinant Biotinylated Cynomolgus SOST/Sclerostin Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Gln69-Tyr258 (accession: XP_005584428.2).

Product details

Molecular aliases: sclerostin; SOST; VBCHsclerosteosis; CDD; DAND6; SOST1; VBCH
Protein length: Gln69-Tyr258
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 24.39 kDa. Due to glycosylation, the protein migrates to 32-38 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Supplied as 0.22 μm filtered solution in PBS, 200mM L-arginine (pH 7.4).
Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Data display

Bis-Tris PAGE Data — Biotinylated Cynomolgus SOST on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

ELISA Data — Immobilized Biotinylated Cynomolgus SOST, His Avi tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human LRP-6, mFc Tag with the EC50 of 37.6ng/ml determined by ELISA. (QC Test)

Background

SOST, also known as sclerostin, is a member of the cerberus/DAN family, a group of secreted glycoproteins characterized by a cysteine-knot motif. SOST is negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation.

References

1.Claire M N, Patton D, Hayes J S. Sclerostin Antibody Therapy for the Treatment of Osteoporosis: Clinical Prospects and Challenges[J]. Journal of Osteoporosis, 2016, 2016:1-22.

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Biotinylated Cynomolgus SOST/Sclerostin Protein

Catalog numberSOST-CB001

HEK293Molecule · sclerostinMolecular weight · 24.39 kDa

Biotinylated Cynomolgus SOST/Sclerostin Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

ELISA

ELISA Data

ELISA

ELISA Data

Background

References

Biotinylated Cynomolgus SOST/Sclerostin Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

ELISA

ELISA Data

ELISA

ELISA Data

Background

References