Biotinylated Human NaPi2b/SLC34A2 Nanodisc

Name: Biotinylated Human NaPi2b/SLC34A2 Nanodisc
Brand: Puweituo Bio
SKU: SLC34A2-HB001

Catalog numberSLC34A2-HB001

HumanHEK293Molecule · NaPi-2bMolecular weight · 88.6 kDa

Product introduction

Recombinant Biotinylated Human NaPi2b/SLC34A2 Nanodisc is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Met1-Leu689 (UniProt accession: O95436-2).

Product details

Molecular aliases: NaPi-2b; NaPi 2b; NAPI-IIb; NPTIIb; NaPi2b; PULAM;
Protein length: Met1-Leu689
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 88.6 kDa.
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Supplied as 0.22 μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.
Storage: Valid for 6 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Negative control: CON-HM1NB

Data display

ELISA Data — Immobilized Biotinylated Human NaPi2b Nanodisc, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Lifastuzumab, hFc Tag with the EC50 of 10.8ng/ml determined by ELISA. (QC Test)

SPR Data — Biotinylated Human NaPi2b Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Lifastuzumab, hFc Tag with an affinity constant of 2.64 nM as determined in SPR assay (Biacore T200).

Background

SLC34A2, a member of the SLC34 family, is a multipass membrane protein and encodes a type 2b sodium-dependent phosphate transporter, NaPiIIb. It is known that SLC34A2 can mediate transporting inorganic phosphate into epithelial cells via sodium ion cotransport. SLC34A2 also strongly inhibited tumor growth and metastasis ability in an A549 subcutaneous tumor model and lung metastasis mode.

References

1.Li Y, Chen X, Lu H. Knockdown of SLC34A2 Inhibits Hepatocellular Carcinoma Cell Proliferation and Invasion. Oncol Res. 2016 Oct 27;24(6):511-519. doi: 10.3727/096504016X14719078133483. PMID: 28281971; PMCID: PMC7838622.

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Biotinylated Human NaPi2b/SLC34A2 Nanodisc

Catalog numberSLC34A2-HB001

HumanHEK293Molecule · NaPi-2bMolecular weight · 88.6 kDa

Product introduction

Recombinant Biotinylated Human NaPi2b/SLC34A2 Nanodisc is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Met1-Leu689 (UniProt accession: O95436-2).

Biotinylated Human NaPi2b/SLC34A2 Nanodisc

Product introduction

Product details

Data display

ELISA

ELISA Data

ELISA

ELISA Data

SPR

SPR Data

Background

References

Biotinylated Human NaPi2b/SLC34A2 Nanodisc

Product introduction

Product details

Data display

ELISA

ELISA Data

ELISA

ELISA Data

SPR

SPR Data

Background

References