Non-biotinylated Human SIRP gamma/CD172g Protein, Ultra Low Endotoxin

Name: Non-biotinylated Human SIRP gamma/CD172g Protein, Ultra Low Endotoxin
Brand: Puweituo Bio
SKU: SIRPG-HP001

Catalog numberSIRPG-HP001

HumanHEK293Molecule · CD172gMolecular weight · 39.7 kDa

Product introduction

Recombinant Human SIRP gamma/CD172g Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Glu29-Pro360 (UniProt accession: Q9P1W8-1).

Product details

Molecular aliases: CD172g; FLJ42230; SIRP beta 2; SIRP gamma; SIRP-B2; SIRPG; Cripto-1; bA77C3.1; SIRP β 2; SIRPγ; SIRP γ
Protein length: Glu29-Pro360
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 39.7 kDa. Due to glycosylation, the protein migrates to 45-52 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
Endotoxin: Less than 0.01 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Human SIRP gamma on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.

SEC-HPLC Data — The purity of Human SIRP gamma is greater than 90% as determined by SEC-HPLC.

ELISA Data — Immobilized Human SIRP gamma, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human CD47, hFc Tag (Cat. CD7-HM247) with the EC50 of 2.46μg/ml determined by ELISA.

Background

Signal regulatory protein gamma (SIRP gamma, designated CD172g), also called SIRP beta 2, is a monomeric 45-47 kDa type I transmembrane protein belonging to the SIRP/SHPS (CD172) family of the Ig superfamily.SIRP gamma is probable immunoglobulin-like cell surface receptor. On binding with CD47, mediates cell-cell adhesion. Engagement on T-cells by CD47 on antigen-presenting cells results in enhanced antigen-specific T-cell proliferation and costimulates T-cell activation.

References

1.Piccio, L. Adhesion of human T cells to antigen-presenting cells through SIRP beta2-CD47 interaction costimulates T-cell proliferation[J]. Blood, 2005, 105(6):2421-2427. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.

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Non-biotinylated Human SIRP gamma/CD172g Protein, Ultra Low Endotoxin

Catalog numberSIRPG-HP001

HumanHEK293Molecule · CD172gMolecular weight · 39.7 kDa

Product introduction

Recombinant Human SIRP gamma/CD172g Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Glu29-Pro360 (UniProt accession: Q9P1W8-1).

Non-biotinylated Human SIRP gamma/CD172g Protein, Ultra Low Endotoxin

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References

Non-biotinylated Human SIRP gamma/CD172g Protein, Ultra Low Endotoxin

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References