Non-biotinylated Human SIRP alpha V8 Protein

Name: Non-biotinylated Human SIRP alpha V8 Protein
Brand: Puweituo Bio
SKU: SIRPA-HP012

Catalog numberSIRPA-HP012

HumanHEK293Molecule · CD172aMolecular weight · 40.1 kDa

Product introduction

Recombinant Human SIRP alpha V8 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Glu31-Arg369(S44L, S50T, I52T, H54R, V57A) (accession: ATD50864.1).

Product details

Molecular aliases: CD172a; BIT; MFR; MYD1; MYD-1; P84; PTPNS1; SHPS1; SHPS-1; SIRP alpha; SIRPA
Protein length: Glu31-Arg369
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 40.1 kDa. Due to glycosylation, the protein migrates to 52-68 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Human Sirpa V8 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Human Sirpa V8 is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Human SIRP alpha V8, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA.

Background

Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal.Cancer cells highly expressed CD47 that activate SIRP α and inhibit macrophage-mediated destruction.

References

1.Weiskopf K, Ring A M, Ho C C M, et al. Engineered SIRP? Variants as Immunotherapeutic Adjuvants to Anticancer Antibodies[J]. Science, 2013, 341(6141):88-91. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.

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Non-biotinylated Human SIRP alpha V8 Protein

Catalog numberSIRPA-HP012

HumanHEK293Molecule · CD172aMolecular weight · 40.1 kDa

Non-biotinylated Human SIRP alpha V8 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References

Non-biotinylated Human SIRP alpha V8 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References