Biotinylated Human Siglec-9 Protein

Name: Biotinylated Human Siglec-9 Protein
Brand: Puweituo Bio
SKU: SIGLEC9-HB001

Catalog numberSIGLEC9-HB001

HumanHEK293Molecule · CD329Molecular weight · 38.9 kDa

Product introduction

Recombinant Biotinylated Human Siglec-9 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Gln18-Gly348 (UniProt accession: Q9Y336-1).

Product details

Molecular aliases: CD329; CDw329; FOAP-9; OBBP-Like; Siglec-9; SIGLEC9; FOAP9
Protein length: Gln18-Gly348
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 38.9 kDa. Due to glycosylation, the protein migrates to 70-90 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22μm filtered solution in 25mM MES, 150mM NaCl (pH 5.5). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in 25mM MES (pH 5.5). Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Biotinylated Human Siglec-9 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Background

Siglec-9 is a sialic-acid-binding lectin expressed predominantly on myeloid cells. Aberrant glycosylation occurs in essentially all types of cancers and results in increased sialylation. Thus, when the mucin MUC1 is expressed on cancer cells, it is decorated by multiple short, sialylated O-linked glycans (MUC1-ST).

References

1.Beatson R, Tajadura-Ortega V, Achkova D, Picco G, Tsourouktsoglou TD, Klausing S, Hillier M, Maher J, Noll T, Crocker PR, Taylor-Papadimitriou J, Burchell JM. The mucin MUC1 modulates the tumor immunological microenvironment through engagement of the lectin Siglec-9. Nat Immunol. 2016 Nov;17(11):1273-1281. doi: 10.1038/ni.3552. Epub 2016 Sep 5. PMID: 27595232; PMCID: PMC5257269.

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Biotinylated Human Siglec-9 Protein

Catalog numberSIGLEC9-HB001

HumanHEK293Molecule · CD329Molecular weight · 38.9 kDa

Product introduction

Recombinant Biotinylated Human Siglec-9 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Gln18-Gly348 (UniProt accession: Q9Y336-1).

Biotinylated Human Siglec-9 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

Background

References

Biotinylated Human Siglec-9 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

Background

References