Biotinylated Cynomolgus Siglec-10 Protein

Name: Biotinylated Cynomolgus Siglec-10 Protein
Brand: Puweituo Bio
SKU: SIGLEC10-CB001

Catalog numberSIGLEC10-CB001

HEK293Molecule · SLG2Molecular weight · 61.71 kDa

Product introduction

Recombinant Biotinylated Cynomolgus Siglec-10 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Thr17-Asn552 (UniProt accession: A0A2K5WBX8).

Product details

Molecular aliases: SLG2; SIGLEC10; MGC126774; PRO940
Protein length: Thr17-Asn552
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 61.71 kDa. Due to glycosylation, the protein migrates to 72-82 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Supplied as 0.22 μm filtered solution in 25mM MES, 150mM NaCl, 0.5M Arginine (pH 5.0).
Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Data display

Bis-Tris PAGE Data — Biotinylated Cynomolgus Siglec-10 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Background

Siglec-10 is a ligand for CD52, the target of the therapeutic monoclonal antibody Alemtuzumab. It is also reported to bind to Vascular adhesion protein 1 (VAP-1) and to the co-stimulatory molecule CD24 also known as HSA (Heat-stable antigen). Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system.

References

1.Barkal AA, et al. CD24 signalling through macrophage Siglec-10 is a target for cancer immunotherapy. Nature. 2019 Jul 31. doi: 10.1038/s41586-019-1456-0.

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Biotinylated Cynomolgus Siglec-10 Protein

Catalog numberSIGLEC10-CB001

HEK293Molecule · SLG2Molecular weight · 61.71 kDa

Biotinylated Cynomolgus Siglec-10 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

Background

References

Biotinylated Cynomolgus Siglec-10 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

Background

References