Biotinylated Human Semaphorin 4A/SEMA4A Protein

Name: Biotinylated Human Semaphorin 4A/SEMA4A Protein
Brand: Puweituo Bio
SKU: SEMA4A-HB001

Catalog numberSEMA4A-HB001

HumanHEK293Molecule · Semaphorin-4AMolecular weight · 75.31 kDa

Product introduction

Recombinant Biotinylated Human Semaphorin 4A/SEMA4A Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Gly33-His683 (UniProt accession: Q9H3S1-1).

Product details

Molecular aliases: Semaphorin-4A; Semaphorin-B; SEMA4A
Protein length: Gly33-His683
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 75.31 kDa. Due to glycosylation, the protein migrates to 85-95 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 0.1 EU per μg by the LAL method.
Formulation: Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Data display

Bis-Tris PAGE Data — Biotinylated Human Semaphorin 4A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Human Semaphorin 4A is greater than 95% as determined by SEC-HPLC.

SPR Data — Human LILRB2, hFc Tag (Cat. LIL-HM2B2) captured on CM5 Chip via Protein A can bind Biotinylated Human Semaphorin 4A, His Tag with an affinity constant of 34.05 nM as determined in SPR assay (Biacore T200).

Background

Neuroimmune semaphorin 4A (Sema4A), a member of semaphorin family of transmembrane and secreted proteins, is an important regulator of neuronal and immune functions. In the nervous system, Sema4A primarily regulates the functional activity of neurons serving as an axon guidance molecule. In the immune system, Sema4A regulates immune cell activation and function, instructing a fine tuning of the immune response.

References

1.Iyer AS, Chapoval SP. Neuroimmune Semaphorin 4A in Cancer Angiogenesis and Inflammation: A Promoter or a Suppressor? Int J Mol Sci. 2018 Dec 30;20(1):124. doi: 10.3390/ijms20010124. PMID: 30598022; PMCID: PMC6337608.

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Biotinylated Human Semaphorin 4A/SEMA4A Protein

Catalog numberSEMA4A-HB001

HumanHEK293Molecule · Semaphorin-4AMolecular weight · 75.31 kDa

Biotinylated Human Semaphorin 4A/SEMA4A Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

SPR

SPR Data

Background

References

Biotinylated Human Semaphorin 4A/SEMA4A Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

SPR

SPR Data

Background

References