Human R spondin 1/RSPO1 Protein

Name: Human R spondin 1/RSPO1 Protein
Brand: Puweituo Bio
SKU: RSPONDIN1-HP001

Catalog numberRSPONDIN1-HP001

HumanCHOMolecule · R-spondin-1Molecular weight · 27.3 kDa

Product introduction

Recombinant Human R spondin 1/RSPO1 Protein is expressed in CHO cells with a His tag at the C-terminus. It contains amino acid residues Arg31-Ala263 (UniProt accession: Q2MKA7).

Product details

Molecular aliases: R-spondin-1; RSPO1; CRISTIN3; FLJ40906; RP11-566C13.1; RSPO; R-Spondin1
Protein length: Arg31-Ala263
Expression system: CHO
Molecular weight: The protein has a predicted MW of 27.3 kDa. Due to glycosylation, the protein migrates to 38-42 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE
Endotoxin: Less than 0.05 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Human R spondin 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

ELISA Data — Immobilized Human R spondin 1, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-R spondin 1 Antibody, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA (QC Test).

BLI Data — Loaded Human R spondin 1, His Tag on Anti-His-Biosensor can bind Human RNF43, hFc Tag with an affinity constant of 18.50 nM as determined in BLI assay.

Cell Based Assay Data — Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 1.0 - 10.0 ng/mL in the presence of 5 ng/mL recombinant Human Wnt Surrogate-Fc Fusion (WNT-HM23A) (QC Test).

Background

The R-spondins are members of a superfamily of thrombospondin type 1 repeat (TSR-1)-containing proteins. The prototype member (discovered in 1971) was isolated from platelets that had been stimulated with thrombin, and was therefore designated 'thrombin-sensitive protein.

References

1.Kamata T, Katsube K, Michikawa M, Yamada M, Takada S, Mizusawa H: R-spondin, a novel gene with thrombospondin type 1 domain, was expressed in the dorsal neural tube and affected in Wnts mutants. Biochim Biophys Acta. 2004, 1676: 51-62.

加载中…

Human R spondin 1/RSPO1 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

ELISA

ELISA Data

ELISA

ELISA Data

ELISA

ELISA Data

BLI

BLI Data

BLI

BLI Data

Bioactivity

Cell Based Assay Data

Background

References

Human R spondin 1/RSPO1 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

ELISA

ELISA Data

ELISA

ELISA Data

ELISA

ELISA Data

BLI

BLI Data

BLI

BLI Data

Bioactivity

Cell Based Assay Data

Background

References