Biotinylated Human/Cynomolgus Activin RIIB/ACVR2B Protein (Primary Amine Labeling)

Name: Biotinylated Human/Cynomolgus Activin RIIB/ACVR2B Protein (Primary Amine Labeling)
Brand: Puweituo Bio
SKU: RIIB-HF003

Catalog numberRIIB-HF003

HumanHEK293Molecule · Activin receptor type-2BMolecular weight · 39.32 kDa

Product introduction

Recombinant Biotinylated Human/Cynomolgus Activin RIIB/ACVR2B Protein (Primary Amine Labeling) is expressed in HEK293 cells with an mFc (IgG1) tag at the C-terminus. It contains amino acid residues Ser19-Thr137 (Human) / Ser40-Thr158 (Cynomolgus) (accession: Q13705-1(Human) / XP_045242398.1(Cynomolgus)).

Product details

Molecular aliases: Activin receptor type-2B; ACVR2B; Activin RIIB; ActRIIB
Protein length: Ser19-Thr137
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 39.32 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Biotinylated Human/Cynomolgus Activin RIIB on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

ELISA Data — Immobilized Human Activin A, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human/Cynomolgus Activin RIIB, mFc Tag with the EC50 of 37.8ng/ml determined by ELISA (QC Test).

Background

ActRIIB (activin receptor type-2B) is an activin receptor subtype constitutively expressed in the whole body, playing a role in cellular proliferation, differentiation, and metabolism. For its various physiological activities, ActRIIB interacts with activin and multiple other ligands including myostatin (MSTN), growth differentiation factor 11 (GDF11), and bone morphogenetic protein 9 (BMP9).

References

1.Sakamoto K, Kanematsu-Yamaki Y, Kamada Y, Oka M, Ohnishi T, Miwa M, Asami T, Inooka H. Identification of ligand-selective peptidic ActRIIB-antagonists using phage display technology. Biochem Biophys Rep. 2017 Jun 10;11:33-39. doi: 10.1016/j.bbrep.2017.06.001. PMID: 28955765; PMCID: PMC5614685.

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Biotinylated Human/Cynomolgus Activin RIIB/ACVR2B Protein (Primary Amine Labeling)

Catalog numberRIIB-HF003

HumanHEK293Molecule · Activin receptor type-2BMolecular weight · 39.32 kDa

Biotinylated Human/Cynomolgus Activin RIIB/ACVR2B Protein (Primary Amine Labeling)

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

ELISA

ELISA Data

Background

References

Biotinylated Human/Cynomolgus Activin RIIB/ACVR2B Protein (Primary Amine Labeling)

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

ELISA

ELISA Data

Background

References