Biotinylated Mouse Qa-1b&B2M&Qdm (AMAPRTLLL) Monomer Protein

Name: Biotinylated Mouse Qa-1b&B2M&Qdm (AMAPRTLLL) Monomer Protein
Brand: Puweituo Bio
SKU: QA1B-HB001

Catalog numberQA1B-HB001

MouseHEK293Molecule · Qdm; Qa-1b; MHC; H2-T23Molecular weight · 50.60 kDa

Product introduction

Recombinant Biotinylated Mouse Qa-1b&B2M&Qdm (AMAPRTLLL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains His23-Pro296(Qa-1b), Ile21-Met119(B2M) and AMAPRTLLL peptide (accession: P06339(Q1-1b) & P01887(B2M) &AMAPRTLLL).

Product details

Molecular aliases: Qdm; Qa-1b; MHC; H2-T23
Protein length: His23-Pro296
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 50.60 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Biotinylated Mouse Qa-1b&B2M&Qdm (AMAPRTLLL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Mouse Qa-1b&B2M&Qdm (AMAPRTLLL) Monomer is greater than 95% as determined by SEC-HPLC.

Background

Qa-1b binds a peptide (AMAPRTLLL), referred to as Qdm (for Qa-1 determinant modifier), derived from the signal sequence of murine class Ia molecules. This peptide binds with high affinity and accounts for almost all of the peptides associated with this molecule. Human histocompatibility leukocyte antigen (HLA)-E, a homologue of Qa-1b, binds similar peptides derived from human class Ia molecules and interacts with CD94/NKG2 receptors on natural killer cells.

References

1.Kurepa Z, Hasemann CA, Forman J. Qa-1b binds conserved class I leader peptides derived from several mammalian species. J Exp Med. 1998 Sep 7;188(5):973-8. doi: 10.1084/jem.188.5.973. PMID: 9730898; PMCID: PMC2213384.

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Biotinylated Mouse Qa-1b&B2M&Qdm (AMAPRTLLL) Monomer Protein

Catalog numberQA1B-HB001

MouseHEK293Molecule · Qdm; Qa-1b; MHC; H2-T23Molecular weight · 50.60 kDa

Biotinylated Mouse Qa-1b&B2M&Qdm (AMAPRTLLL) Monomer Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

Background

References

Biotinylated Mouse Qa-1b&B2M&Qdm (AMAPRTLLL) Monomer Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

Background

References