Biotinylated Cynomolgus PSMA/FOLH1 Protein (active dimer)

Name: Biotinylated Cynomolgus PSMA/FOLH1 Protein (active dimer)
Brand: Puweituo Bio
SKU: PSMA-CB001

Catalog numberPSMA-CB001

HEK293Molecule · GCP2Molecular weight · 83.34 kDa

Product introduction

Recombinant Biotinylated Cynomolgus PSMA/FOLH1 Protein (active dimer) is expressed in HEK293 cells with a His tag and Avi tag at the N-terminus. It contains amino acid residues Lys44-Ala750 (UniProt accession: A0A2K5VNZ0).

Product details

Molecular aliases: GCP2; PSMA; PSMAFGCP; FGCP; Folate hydrolase 1; FOLHNAALADase I; GCPII; GCPIImGCP; NAALAD1; NAALADase I; NAALAdase; FOLH1; GIG27; PSM; FOLH; mGCP
Protein length: Lys44-Ala750
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 83.34 kDa. Due to glycosylation, the protein migrates to 100-130 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 0.1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22 μm filtered solution in 50mM MES, 150mM NaCl, 8% trehalose (pH 6.0).
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in 50mM MES (pH 6.0). Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Biotinylated Cynomolgus PSMA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Cynomolgus PSMA is greater than 95% as determined by SEC-HPLC.

SEC-MALS Data — The purity of Biotinylated Cynomolgus PSMA is greater than 95% and the molecular weight of this protein is around 180-220 kDa as determined by SEC-MALS.

ELISA Data — Immobilized Biotinylated Cynomolgus PSMA, His Avi tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-PSMA Antibody, hFc Tag with the EC50 of 1.8ng/ml determined by ELISA. (QC Test)

Background

Prostate-specific membrane antigen (PSMA) is an enzyme that in humans is encoded by the FOLH1 (folate hydrolase 1) gene, also known as Glutamate carboxypeptidase II (GCPII). Human PSMA is highly expressed in the prostate, roughly a hundred times greater than in most other tissues. In some prostate cancers, PSMA is the second-most upregulated gene product, with an 8- to 12-fold increase over levels in noncancerous prostate cells.

References

1.Song C, Francesca Z, Williams D G, et al. Antitumor Activity of MEDI3726 (ADCT-401), a Pyrrolobenzodiazepine Antibody-drug Conjugate Targeting PSMA, in Pre-clinical Models of Prostate Cancer[J]. Molecular Cancer Therapeutics, 2018:molcanther.0982.2017-.

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Biotinylated Cynomolgus PSMA/FOLH1 Protein (active dimer)

Catalog numberPSMA-CB001

HEK293Molecule · GCP2Molecular weight · 83.34 kDa

Biotinylated Cynomolgus PSMA/FOLH1 Protein (active dimer)

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

SEC-MALS

SEC-MALS Data

ELISA

ELISA Data

Background

References

Biotinylated Cynomolgus PSMA/FOLH1 Protein (active dimer)

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

SEC-MALS

SEC-MALS Data

ELISA

ELISA Data

Background

References