Synonym
Hamp1; Hepc; Hepc1; HFE2B; LEAP1; LEAP-1; PLTR
Source / expression
Recombinant Human Hepcidin/HAMP Protein is expressed from E.coli with GST tag at the N-Terminus.It contains Asp60-Thr84 [Accession | P81172].
Molecular weight
The protein has a predicted MW of 29.08 kDa same as Bis-Tris PAGE result.
Endotoxin
Less than 1 EU per μg by the LAL method.
Purity
> 95% as determined by Bis-Tris PAGE
Formulation
Lyophilized from 0.22μm filtered solution in 50mM Tris-HCl, 150mM NaCl, 2mM DTT (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Storage
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
