分子别名 (Synonym)
Hamp1; Hepc; Hepc1; HFE2B; LEAP1; LEAP-1; PLTR
来源/表达系统 (Source)
Recombinant Human Hepcidin/HAMP Protein is expressed from E.coli with GST tag at the N-Terminus.It contains Asp60-Thr84 [Accession | P81172].
分子量
The protein has a predicted MW of 29.08 kDa same as Bis-Tris PAGE result.
内毒素 (Endotoxin)
Less than 1 EU per μg by the LAL method.
纯度 (Purity)
> 95% as determined by Bis-Tris PAGE
制剂 (Formulation)
Lyophilized from 0.22μm filtered solution in 50mM Tris-HCl, 150mM NaCl, 2mM DTT (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
重构方法 (Reconstitution)
Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
存储 (Storage)
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.