Synonym
Abrineurin; BDNF; ANON2; BULN2
Source / expression
Recombinant Human/Mouse/Rat BDNF Protein is expressed from E.coli without tag. It contains His129-Arg247(Human) / His131-Arg249(Mouse/Rat) [Accession | P23560-1(Human) / P21237-1(Mouse) / P23363(Rat)].
Molecular weight
The protein has a predicted MW of 13.51 kDa. The protein migrates to 15-19 kDa based on Bis-Tris PAGE result.
Endotoxin
Less than 0.01 EU per μg by the LAL method.
Purity
> 95% as determined by Bis-Tris PAGE
Formulation
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Storage
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
