分子别名 (Synonym)
Abrineurin; BDNF; ANON2; BULN2
来源/表达系统 (Source)
Recombinant Human/Mouse/Rat BDNF Protein is expressed from E.coli without tag. It contains His129-Arg247(Human) / His131-Arg249(Mouse/Rat) [Accession | P23560-1(Human) / P21237-1(Mouse) / P23363(Rat)].
分子量
The protein has a predicted MW of 13.51 kDa. The protein migrates to 15-19 kDa based on Bis-Tris PAGE result.
内毒素 (Endotoxin)
Less than 0.01 EU per μg by the LAL method.
纯度 (Purity)
> 95% as determined by Bis-Tris PAGE
制剂 (Formulation)
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
重构方法 (Reconstitution)
Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
存储 (Storage)
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.