Synonym
SHH; Sonic Hedgehog; HHG-1; Sonic hedgehog; HLP3; HPE3; SMMCI; TPT; TPTPS
Source / expression
Recombinant Human Sonic Hedgehog (Shh) Protein is expressed from E.coli without tag.It contains Cys24-Gly197 [Accession | Q15465].
Molecular weight
The protein has a predicted MW of 19.79 kDa kDa. The protein migrates to 23-25 kDa based on Bis-Tris PAGE result.
Endotoxin
Less than 0.05 EU per μg by the LAL method.
Purity
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, 300mM NaCl (pH 7.4). Normally 8% trehalose / 8% mannitol is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Storage
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
